Breeding
Breeding and housing of mice are performed either at iTL and Stony Brook University’s joint specific pathogen free (SPF) mouse facility or in collaboration with Charles River Laboratory’s SPF facility and virus antibody free (VAF+) animals. iTL has relevant national and international accreditations to house and export animals and our SPF status conform to the requirements of major national and international research institutes and companies.
Our breeding services for your mice save you time, space and can simplify all aspects of managing your mouse model colonies. iTL breeds, maintains, and ships your custom model(s) to you to meet your needs.
iTL Delivers additional breeding services after the generation of your custom mouse model
- Maintaining custom model stock
- Generation of homozygous lines
- Cryopreservation and rederivation
Below is an example of a normal post-natal development timeline of a knockout or knockin mouse model.
0 Day Old: Blood red; possible milk spot
1 Day Old: Lighter color red; milk spot present
2 Day Old: Ears appear as nubs; pigment may appear in some strains
3 Day Old: Ear flap start to come away from head (one or both)
4 Day Old: Ears fully developed; completely off head; ears may migrate towards back
5 Day Old: Ears all back; skin appears thicker with more color density
6 Day Old: Milk spot disappearing/gone; skin still shows; colored fuzz behind ears or on neck
7 Day Old: Colored fuzz starts to cover pup; skin does not show
8 Day Old: Belly starts to show fur; lower incisors show but not erupted
9 Day Old: Fur is thicker; females may show inguinal nipples (five pairs of mammaries)
10 Day Old: Lower incisors erupted
11 Day Old: Upper incisors erupted
13 Day Old: Eyes open
(development calendar adapted from Jackson Laboratory)
