iTL specializes in the creation of custom-made mouse knockout (conventional, conditional) and knockin (point mutation,human cDNA replacement,reporter gene) models. In addition, many investigators create their own targeting constructs and have iTL perform the remaining steps, i.e. from electroporation onward. ITL is also capable of creating just targeting vectors, for investigators who want to use another source for the rest of the process.

In 2005, iTL introduced their Hybrid 129SvEv x C57BL/6 line. This line exhibits a significantly higher rate of homologous recombination in the production of mouse knockout and knockin models. The Hybrid 129SvEv x C57BL/6 has shown an average 200% increase in the frequency of positive clones per targeting event than other cell lines used at iTL. These characteristics have resulted in reducing the time involved for electroporation and screening tasks, enabling delivery of mouse models with guaranteed germline transmission to be achieved in as little as 7 months. Another benefit iTL offers with this cell line is the ability to provide scientists with knockout or knockin mouse models at lower cost.

The Hybrid 129SvEv x C57BL/6 line is the third ES cell line developed by iTL and is designed to incorporate the strengths of its 129SvEv and C57BL/6 ES lines. The 129SvEv line exhibits a consistent rate of homologous recombination for efficient germline transmission while enabling the ability to analyze resulting chimeras by coat color. The C57BL/6 line is useful for its physiological relevance to certain genetically-linked health conditions, its many parallels between mouse and human genes, the accuracy of published databases for C57BL/6 genes, and the ability to obtain a mouse on a pure background.