Genotyping
At iTL, we are pleased to provide a number of genotyping solutions for your genetic modified animals. Our services facilitate and accelerate your research by providing fast and accurate mouse genotyping services.
PCR Assay Screening
DNA extractions from clones are used as target templates. Target specific primers allow detection of either the transgence or a knockout gene. The PCR reaction results in amplification of expected size fragments allowing for the determination of the genetic nature of the clones.
Southern Blot Screening
Clone DNA is digested with restriction enzymes to produce fragments which are separated using an agarose gel and blotted onto a specialized membrane. This membrane is hybridized with custom probes designed for your gene target(s). Signal and image development provides a banding pattern to analyze for confirmation of correct vector integration.
Each service has two levels – basic and customized and both include
- DNA quality control check
- Detailed reports of genotyping strategy and results
- Database access to review and download reports
- Delivery of DNA tail samples
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Our Basic services are pre-validated assays for identifying heterozygotes, homozygotes, introduced mutations, and any other genetic modifications.
