CRISPR Mouse Models
At ingenious, our mission is to successfully generate your gene targeted mouse models as quickly as possible without sacrificing quality. Based on these goals, we apply CRISPR and other technologies across our ever expanding portfolio of unique project types and proprietary design options.
CRISPR via Microinjection
We offer CRISPR microinjection services to generate mouse models at reduced cost in less time. By introducing CRISPR/Cas9 along with gRNA(s) and template DNA into zygotes, we are able to generate models quickly and efficiently. At ingenious, we will work with you to design and customize your genetically engineered animal model(s) to meet your exact specifications using our CRISPR-Cas 9 technologies. The ingenious process for generating your custom mouse model is broken down into 3 steps:
1st Step: Strategy Design
Selection of target site, assays, and oligonucleotides, as well as predicted off-target sites. Preparation of sgRNA and Cas9 mRNA.
2nd Step: Production of Founder Mice
Injection of sgRNA and Cas9 mRNA into fertilized embryos, to generate F0 mutated candidate animals. Breeding and genotyping for the presence of the desired mutated candidate F0 animals.
3RD STEP: BREEDING OF FOUNDERS TO OBTAIN F1 MICE
We will breed the new founders to wildtype mice of matching strain background, and genotype their offspring to obtain F1 mice bearing the knockout or knockin allele. Optional breeding will be offered to segregate off-target mutations if necessary.
After You Receive Your Mice
Combining your CRISPR targeted model with additional mouse lines to generate experimental cohorts can become challenging. This may require multiple mating steps, specific genotyping, and calculated colony scale up. ingenious’ post project support services provides the expertise needed for cohort development, as well as cryopreservation services to protect your investment.
Breakthrough in CRISPR Technology
“We found iTL’s service to be exceptionally efficient and very well managed. We generated several mouse lines with iTL, and every step for each project was performed quickly and precisely, without any delays or problems.”
-Santos Franco, PhD