As technology and research progress, ingenious continues to provide clients with knockin mice that meet even the most intricate design requirements. With a design toolkit that includes CRISPR via microinjection, CRISPR Assisted homologous recombination, plasmid vectors, fosmid vectors, and BAC vectors, there is no knockin design that is out of reach. ingenious provides complimentary design consultation from industry veterans for all those considering a genetically modified mouse model project.
Here are some of the knockin mouse models we can develop for you:
With the development of CRISPR, the introduction of point mutations to endogenous genes has never been faster or simpler. If your project requires single or multiple point mutations, across regions big or small, ingenious has the experience and tools for the project.
This includes cDNA’s (human or murine) and/or reporter genes or even entire human genes. Cassettes may be inserted either to replace an endogenous mouse gene or to be fused to the endogenous gene for co-expression. In addition, to facilitate co-expression of a cassette with an endogenous mouse gene, a 2A peptide strategy can be applied. The Rosa26 locus can be utilized for insertion of cassettes if the endogenous gene should not be targeted. This enables inducible overexpression.
Utilizing minigene or inversion design options, ingenious can offer tissue specific expression control over your point mutation within endogenous mouse genes via Cre recombinase. Beyond expression control, these designs reduce the risk of lethal phenotypes normally posed by constitutive expression models.
Tissue specific expression control via Cre Recombinase is available for cassettes of all types. Whether the project requires cDNAs, reporter genes, tags, or a unique custom expression cassette, ingenious can provide a design to meet your needs.
ingenious’ TruHumanization™ service allows for the complete replacement of endogenous mouse genes with human genomic gene sequence including introns, exons, regulatory features, and more. Leave nothing behind.
Inducible Rosa26-Express Targeting™
Tissue specific and doxycycline inducible/reversible expression control over your cDNA or multi component cassette has never been simpler. ingenious’ proprietary Inducible Rosa26-Express Targeting™ system provides a straightforward design option for those who require additional expression control for their research.
A proprietary cassette is inserted into the target gene, producing a mouse model with the potential for five or more versatile functionalities, such as: global gene knockout, tissue / time specific rescue, ectopic expression, inducible/reversible overexpression or knockdown/knockout. Read more about our F.A.S.T.™ technology!
After You Receive Your Mice
Crossing your knockin mice with additional mouse lines to generate experimental cohorts can become challenging, especially when 3 or more lines are involved. ingenious’ post project support services aim to make the process easy and straightforward. Utilizing our proprietary prediction and modeling software, our team of mouse model experts can generate the cohorts you need when you need them and within the timelines and costs predicted.