• Targeted gene knockout mice with multiple options for strategy.
• Conditional knockouts for extra flexibility – take control over timing and affected tissues.
• Delete as little as 200bp, or as much as 200kb – using our BAC technology.
• Enhance the versatility of your model – by combining reporter gene expression with your targeted knockout.
Our experienced scientists listen, care, and deliver.
ingenious Targeting Labs began as one of the first pioneering companies delivering knockout mice. From conventional deletions to conditional knockouts and large scale deletions using BACs, we have developed the technology and expertise to target even the most challenging genes. Working with ingenious, our clients have comfort in knowing that they have chosen a company with longevity, backed by almost two decades of successful, published experience.
Utilizing both CRISPR and ES cell technologies, ingenious offers the following mouse knockout model specialties:
The target region to delete is replaced with a Neomycin selection cassette, or with a reporter gene. The targeted gene is thus inactivated at all times, in all tissues. Conventional knockouts can be created with CRISPR/Cas9 to reduce costs and shorten timelines. Alternately, choose traditional homologous recombination in ES cells for the knockout mice you need without any off-target effects.
Large Scale Deletion
Base your designs around experiments, not limitations. Our proprietary BAC technology enables the specific deletion of very large genomic sequences, such as multiple genes or whole gene clusters, in a single targeting step. By innovating the design and construction of these once-formidable targeting projects, ingenious has drastically reduced the timelines and costs associated with creating these knockout mice. At ingenious, 200 kb is the new standard.
The target region is flanked by loxP sites (“floxed”) to facilitate Cre recombinase-mediated excision, leading to tissue specific gene inactivation. Conditional knockouts have mostly replaced conventional knockouts as they don’t carry the risk of embryonic lethality. In addition, the Cre-loxP system allows researchers to develop much more versatile knockout mice. The parental floxed mouse line can be mated to different Cre or CreERT2 lines if desired. Global Cre expression will still allow for a total body knockout.
Combining speeds normally associated with CRISPR/Cas9 and the reliability of traditional targeting via homologous recombination in ES cells, ingenious’ CRISPR-assisted knockout service provides you with the best that both technologies have to offer. By utilizing the CRISPR/Cas9 system to increase targeting vector integration efficiency, we are able to reduce the time and cost associated with vector construction and ES cell screening while still maintaining targeting quality.
After You Receive Your Mice
Breeding your knockout mice with additional targeted models to generate experimental cohorts can be complex. ingenious’ post project support services aim to make things simple. Utilizing our proprietary prediction and modeling software, our team of breeding veterans build plans of action and generate the cohorts you need, when you need them and within projected timelines and costs.