After You Receive Your Mice

Post Project Services from GeneTargeting.comOnce you receive your mouse model from ingenious, your ultimate goal will be to generate a cohort of mice of a certain age and sex, which you can then utilize for your experiments. You may continue to need more colonies as your experiments evolve. Depending on the model we have generated for you, this may require multiple mating steps, genotyping for various genetic elements, and calculated scaling up to obtain the specific number of mice with the appropriate sex, background and genotype.

We know from talking with many of our clients that traditional in-house breeding often becomes an unnecessarily expensive and time consuming process, which can even lead to the loss of the mouse line. ingenious offers downstream services to make this important endeavor seamless.

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Additional Mating and Cohort Generation

By outsourcing the generation of mouse cohorts to ingenious, you will benefit from expert staff that will work with you to determine the most efficient breeding plan to meet your research goals. Utilizing our proprietary breeding software allows us to develop plans of action based on time lines and costs that best fit your lab. Our service includes:

  • A free online tool to generate an estimated mating plan (coming soon).

  • Breeding small or large cohorts of heterozygous or homozygous mice.

  • Breeding with any Cre lines (or tTA, tTS, CreERT etc.) with your homozygous mice that we can obtain together through ingenious’ relationship with The Jackson Laboratory.

  • Genotyping of all offspring with established protocols developed by ingenious.

  • Timed deliveries at your request between your control and experimental groups.

  • Alleviate the need to justify animal use protocol numbers.

Please contact us to discuss the different cohort development options and to receive a quote.

Cryopreservation

Protect your mouse model and your research investment! We offer sperm cryopreservation and embryo cryopreservation services, various storage plans for cryopreserved sperm and embryos, and cryorecovery service to rederive your mouse line.

Sperm Cryopreservation

The service includes sperm collection and cryopreservation from two male mice, including motility test of thawed sperm. Service also includes in vitro fertilization and culturing of embryos to the blastocyst stage to confirm fertility of cryopreserved sperm.

Embryo Cryopreservation

This service includes sperm collection from two male mice, and subsequent in vitro fertilization of oocytes from superovulated wildtype females. Embryos will be cryopreserved. Service also includes quality control by culturing of thawed embryos to confirm viability.

Note: Embryos will be a mix of wildtype and heterozygous genotypes when heterozygous males are used (the resulting pups must be genotyped), or all embryos will be heterozygous when homozygous males are used.

Tissue Harvest

At ingenious we can harvest all major organs from your custom mouse model, embed or freeze them, and send them to you for your studies.

Colony Management Consultation at Your Own Facility

From the U.S. to Canada, Europe, Asia, and beyond, ingenious now offers over 18 years of colony management expertise, right in the comfort of your own facility. Utilizing proprietary modeling and prediction software, our mouse colony veterans provide the guidance and tools needed to optimize your colony. Whether you are starting a new colony, maintaining an existing colony, or building experimental cohorts, ingenious plots your course to success, making the management of your mouse lines easy and straight-forward. Service benefits include:

  • A service designed to work with your lab personnel.

  • Colony optimization and cost savings.

  • Colony status at a glance.

  • Predictable experimental animal numbers.

  • Full control over your experiments.

  • Preventing the brain drain.

For more information, visit Colony Management Consultation with ingenious

Please contact us to discuss any of our Post Project Services.