Other Useful Resources

Other Useful Resources from GeneTargeting.comWorking with genetically engineered mouse models is a time consuming process – from the planning stage for a mouse model, to actual phenotyping experiments and writing publications and grants. Below we have gathered a few useful resources to help navigate some of these aspects.

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Transgenic Mouse Repositories

Cre transgenic mice are available at the following institutions:

 

CREATE
This organization coordinates resources for conditional expression of mutated mouse alleles.

 

JAX Cre Strains
The Jackson Laboratory offers over 300 cre tool strains.

 

MGI Recombinase Activity
This is a link to the Cre database at Jackson Lab’s MGI site. Mouse Genome Informatics (MGI) offers information on mouse genetic markers, molecular segments, phenotypes, comparative mapping data, experimental mapping data, and geographical displays for genetic, physical, and cytogenetic maps.

 

MMRRC
MMRRC is a national network of breeding and distribution facilities that serve as NIH’s repository of spontaneous and induced mutant mouse and cell lines.

 

Center for Animal Resources and Development
CARD is an international repository and distribution center for genetically engineered mouse models, based in Japan.

 

Canadian Mouse Mutant Repository
Located at the Toronto Centre for Phenogenomics, the CMMR is a central repository for cryopreserved mouse ES cells, sperm, and embryos. Somatic tissue from various established mouse models are available from the CMMR archive in a variety of formats (frozen, fixed, embedded, and glass-slide mounted).

 

Cre-X-Mouse
This is a database, maintained by Dr. Nagy’s laboratory at Mount Sinai Hospital, of Cre transgenic mouse lines.

 

CreZoo
This resource is a European based, virtual repository of Cre information.

 

EMMA
EMMA is a non-profit repository for the collection, archiving (via cryopreservation) and distribution of mutant mouse strains for biomedical and preclinical research.

 

ICS Mouse Cre
This is a CreERT2 database from the Institut Clinique de la Souris (ICS).

 

IMSR
The IMSR is an online database of mouse model strains, stocks, and mutant ES cell lines.

 

National Cancer Institute Mutant Resource
The NCI provides live colonies and cryopreserved mouse cancer models and associated strains for scientific research.

 

RIKEN BioResource Centre
Based in Japan, BRC collects, preserves, and distributes mouse model strains to the scientific community.

Mouse Breeding Resources

Charles River

Charles River offers their Guidebook on Mouse and Rat Colony Management to interested researchers. Email askcharlesriver@crl.com to request a copy.

 

Jackson Laboratory

JAX focuses solely on mouse breeding and colony maintenance. Besides an overview of mouse reproductive biology, it also includes detailed information on specific JAX substrains. Find access to this manual here.

Mouse Genetics Communities and Forums

The goal of these forums is knowledge exchange. Anyone who signs up can ask or answer questions.

Transgenic List (tg-l)
The Transgenic List is an e-mail discussion forum hosted by the ISTT. It serves those with specialized interests in genetic modifications of experimental laboratory animals.

 

Mouse Genome Informatics List (mgi-l)

The mgi list is an e-mail discussion forum offered by Jackson Laboratory. It serves those with a specialized interest in mouse genetics. Mouse Genome Informatics (MGI) offers information on mouse genetic markers, molecular segments, phenotypes, comparative mapping data, experimental mapping data, and geographical displays for genetic, physical, and cytogenetic maps.

 

International Society for Transgenic Technologies (ISTT)

The ISTT is an international society dedicated to the education about, sharing and advancement of Transgenic technology.

Bioinformatics

ENSEMBL mouse genome browser

The Ensembl project produces genome databases for vertebrates and other eukaryotic species, and makes this information freely available online. The link provided on this page, is for the mouse genome database.

 

UCSC Genome Bioinformatics

This site contains the reference sequence and working draft assemblies for a large collection of genomes, and provides genome browser, gene sorter, blast search function, and publications

 

The Sequence Manipulation Suite

The Sequence Manipulation Suite is a collection of JavaScript programs for generating, formatting, and analyzing short DNA and protein sequences. It is commonly used by molecular biologists, for teaching, and for program and algorithm testing.

Colony Management Software

Mouse colony management systems have revolutionized labs, allowing researchers to shift their focus away from colony management by streamlining workflow and reducing human error, freeing up time, money and potentially space. Here are several commercially available colony management softwares:

Soft Mouse

SoftMouse Internet Colony Management System tracks breeding, pedigrees, cages, mice, litters, genotypes, mouselines, transgenic, etc. The system is ideal for animal technicians and biomedical researchers in small and large laboratories.

 

Mosaic Vivarium

Mosaic Vivarium helps facility managers and investigators make timely, intelligent and cost-saving decisions about laboratory animal resources. Get instant answers on cage density, mating productivity, colony aging, IACUCcounts, per diems and more.

 

Lab Guru

Lab Guru tracks your entire rodent research story, from strains to specimens to cages. Integrate with your research by linking rodents to related projects, storage locations, materials, experiments and results. Schedule and plan experiments easily. Receive reminders for maintenance tasks.

 

mLIMS

mLIMS is a web-based animal colony management system. It helps researchers to improve efficiency and save animal costs dramatically. mLIMScomes with real-time summaries, auto-reminders for animal events, powerful search function, and advanced tools for cage cards, pedigree, mating and litter operations. It’s 100% web-based, easy to use, and perfect for managing animal data within a research group.

 

JAX Colony Management System

The JAX Colony Management System (JCMS) is a free and community supported multi-user relational database system for managing research mouse colonies. It has a broad user-community with over 4,500 people currently signed up on the JCMS user support list. Newly released JCMS Web runs on any platform and most modern web browsers.

General Mouse Resources

HistoWiz

HistoWiz is a histology service company that processes, embeds, cuts, stains tissue specimens and digitizes the results for biomedical researchers……with a guaranteed 3 day turnaround-time. From wet tissue to our Cloud based histology image database, long-term slide archival, automated image analysis and mouse cancer pathology expertise, we keep your research data at your fingertips.

Histowiz logo from GeneTargeting.com

 

3R’s – Replacement, Refinement, and Reduction of Animals in Research

The 3R’s were developed to promote humane animal research. This includes minimizing the use of animals in research and improving animal welfare to reduce pain and suffering. The 3R’s have resulted in policy and regulatory changes as well as the development off new technology and approaches.

 

German Mouse Clinic

Offers standardized phenotypic screens with over 550 parameters for a variety of disciplines. The mouse phenotyping service is provided at no charge but in turn all findings and results are jointly owned by the researcher and the GMC with the goal to provide an open access platform for the scientific community.

 

International Mouse Phenotyping Consortium
An international collaboration between centers in Europe, North America, Asia and Australia; aimed at offering an exhaustive list of phenotyping screens to enrich individually produced datasets, and generating a central, publicly accessible database of gene-associated phenotypes.

 

Jackson Labs Mice Database Query
Comprehensive query form to aid in identifying existing mice by research field, new models for distribution, and strains just released from development.

 

The Mouse Phenome Database
The database is a standardized collection of protocols and reference data from an international collaboration of scientists. It can be used, to find phenotypic measurements for specific strains, to find strains that meet specific phenotypic criteria, or to explore phenotype/genotype correlations.

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