Conventional vs. Conditional Knockout Mice – The Superiority of Advanced Conditional Knockout Technologies
There are many reasons why the issue of conventional vs. conditional knockout mouse models is solved through a clear superiority of advanced conditional methods. Traditional knockouts have long been considered the most advanced method of engineering knockout laboratory mice. Some of the latest techniques in use today, however, allow for far greater accuracy and efficiency when it comes to ensuring that a greater number of mouse embryos survive. Also, they are ideal for ensuring that many of the most common limitations of conventional knockout techniques can be bypassed entirely.
The Basics of Conventional and Conditional Knockouts
When it comes to comparing conventional vs. conditional knockout methods, it helps to know the basics. While conventional knockouts were first, involving animal models created with artificially impaired or eliminated genes that are applied to all the tissues of their bodies, conditional knockouts are more advanced, involving gene knockouts that only target specific tissues or organs. For example, in order to study a disease that affects the liver, scientists might use a conditional knockout that eliminates a specific gene only in that specific organ. Their results can then become much more accurate in terms of the drugs and genetic therapies they unveil, which could save countless lives in the long run.
The Limitations of Traditional Conventional Knockout Methods
In terms of understanding the inferiority of conventional vs. conditional knockout models, it’s important to remember that conventional knockouts are far less efficient and take much longer to put into practice. Because of the fact that in a conventional knockout, researchers start from a blastocyst and the mice that are born will contain transgenic changes in every cell, chimeras can be born, and the process takes a greater amount of time. Also, a traditional knockout makes it much harder to find the clones that possess the transgene at all, which makes the process more difficult as well.
Why Are New Conditional Knockout Techniques so Popular?
When considering conventional vs. conditional knockouts, it’s important to realize that, while conditional knockouts started in a similar fashion, the techniques and technologies used to make them possible already had to be more advanced than the ones used for conventional knockouts from the very start. Today, advanced technologies such as CRISPR can be used to have zygotes injected directly, eliminating the random, time-consuming and uncertain results you get from traditional knockout methods and ensuring that the process of generating conditional knockouts can be made to be much more straightforward. Researchers are able to use a lower number of zygotes to get viable results, and sometimes homozygous knockouts can be found right from the start.
The Advantages of Cutting Edge Gene Editing with Conditional Knockouts
More than four years after the initial development of the CRISPR/Cas9 technique, this and other advanced technologies for obtaining conditional knockouts have become more popular and widely used than conventional knockout techniques. Newer technologies allow for simplicity and efficiency, diminishing the time required to obtain modified target genes and enhancing the bioinformatics tools of the past to identify appropriate RNA sequences. When it comes to the battle between conventional vs. conditional knockout models, it’s safe to say that the efficiency of new technological knockout methods favor the easier and more result-driven conditional knockout methods, to the older, traditional techniques.