CRISPR Mouse Models
ingenious is a proven leader in developing and deploying the latest technologies for creating gene targeted mouse models. Contact us today to find out how we can apply the power of CRISPR/Cas9 for your next project, whether it is a CRISPR knockout, gene mutation, or small sequence insertion.
Available CRISPR Model Types:
Cas9 makes targeted cuts in your gene of interest which are repaired by an error-prone DNA repair pathway. Mutations introduced by this process disrupt the gene, to create a knockout allele.
A small number of nucleotide changes are introduced at a target site by combining CRISPR/Cas9 with a repair oligo. Introduce a disease-causing mutation or humanize a critical amino acid with this strategy.
Small Sequence Insertion
Insert up to 90 bases of new sequence at a single site, for example add an epitope tag to label and purify your protein of interest.
THE CRISPR/CAS9 PROCESS
The use of CRISPR/Cas9 to introduce targeted DNA modifications builds on decades of research into bacterial biology and DNA repair mechanisms. With this exciting new technology scientists at ingenious can generate mouse models at reduced cost in less time. Targeting materials are injected directly into embryos, bypassing the need to use ES cells when making complex models.
At ingenious, we work with you to design and customize your genetically engineered animal models, including CRISPR knockout, point mutation, or small sequence insertion models. We will meet your exact specifications using our CRISPR/-Cas-9 technologies.
The ingenious process for generating your custom mouse model utilizes 3 steps:
1st Step: Strategy Design
Selection of target site, assays, and oligonucleotides, as well as predicted off-target sites. Preparation of sgRNA and Cas9 mRNA.
2nd Step: Production of Founder Mice
Injection of sgRNA and Cas9 mRNA into fertilized embryos, to generate FO mutated candidate animals. Breeding and genotyping to identify FO animals with the desired mutation.
3RD STEP: BREEDING OF FOUNDERS TO OBTAIN F1 MICE
The new founders are bred with wildtype mice of matching strain background, and their offspring genotyped to identify F1 mice bearing the knockout or knockin allele.
After You Receive Your Mice
Combining your CRISPR targeted model with additional mouse lines to generate experimental cohorts can become challenging. This may require multiple mating steps, specific genotyping, and calculated colony scale up. ingenious’ post project support services provides the expertise needed for cohort development, as well as cryopreservation services to protect your investment.
Breakthrough in CRISPR Technology
“We found iTL’s service to be exceptionally efficient and very well managed. We generated several mouse lines with iTL, and every step for each project was performed quickly and precisely, without any delays or problems.”
-Santos Franco, PhD