Targeted ES Cell Lines
The International Knockout Mouse Consortium (KOMP, EUCOMM) is an important resource for targeting constructs, targeted embryonic stems cells, and targeted mice. If mice do not yet exist for a gene of interest, we can work with either the targeting vector or the targeted ES cell clones from the consortium to generate mice. The following service options are available:
Working with a Targeting Vector from the Consortium
We can generate targeted ES cell lines and targeted mice from KOMP/EUCOMM targeting vectors. We electroporate your chosen construct into our ES cells and screen the resulting clones using PCR and Southern Blotting. Once positive clones are identified and confirmed via Southern blotting, we guarantee germline transmission mice as long as positive clones are injected at our facility.
Our ES cell lines have a > 90% euploidy rate, compared to the 70% industry standard. From this, over 1,700 gene targeted mouse models have been created and delivered, many of which are published in the high-impact journals Science, Nature, and Cell.
Protect your investment with ingenious’ guarantee of germline transmission. Search now for your KOMP/EUCOMM targeting vector.
Working with Targeted ES Cell Clones from the Consortium
We suggest purchasing 3-4 individual clones if possible and performing essential quality control steps to ensure the clones are properly targeted and have high percentage euploidy. We can provide karyotyping and screening services, or alternatively, those steps can be completed by the client. Confirmed, high quality clones should be selected for blastocyst injections to generate chimeras, and subsequently the germline confirmed F1 mice.
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“iTL recently produced a new knockout model for us from a KOMP targeting vector (knockout first, conditional potential). The entire process was extremely efficient and the staff at iTL were both knowledgeable and helpful. We chose iTL because of their cost-effective service and their guarantee of germline transmission when using a KOMP targeting vector. Now that the project is complete, I would definitely use their services again in the future and would recommend them highly to anyone looking to create a new mouse model.”
-Morgan Fullerton, PhD