KOMP/EUCOMM

Targeted ES Cell Lines

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ingenious’ KOMP/EUCOMM Mouse Models

The International Knockout Mouse Consortium (KOMP, EUCOMM) is an important resource for targeting constructs, targeted embryonic stems cells, and targeted mice. If mice do not yet exist for a gene of interest, we can work with either the targeting vector or the targeted ES cell clones from the consortium to generate mice. The following service options are available:

 

Working with a Targeting Vector from the Consortium

We can generate targeted ES cell lines and targeted mice from KOMP/EUCOMM targeting vectors. We electroporate your chosen construct into our ES cells and screen the resulting clones using PCR and Southern Blotting. Once positive clones are identified and confirmed via Southern blotting, we guarantee germline transmission mice as long as positive clones are injected at our facility.

Our ES cell lines have a > 90% euploidy rate, compared to the 70% industry standard. From this, over 1,700 gene targeted mouse models have been created and delivered, many of which are published in the high-impact journals Science, Nature, and Cell.

Protect your investment with ingenious’ guarantee of germline transmission. Search now for your KOMP/EUCOMM targeting vector.

 

Working with Targeted ES Cell Clones from the Consortium

We suggest purchasing 3-4 individual clones if possible and performing essential quality control steps to ensure the clones are properly targeted and have high percentage euploidy. We can provide karyotyping and screening services, or alternatively, those steps can be completed by the client. Confirmed, high quality clones should be selected for blastocyst injections to generate chimeras, and subsequently the germline confirmed F1 mice.



Cre-Lox Facts

6 Facts You May Not Know About Cre-Lox By Richard Row, PhD The basics of conditional knockout mice are familiar to any researcher who uses mouse models, even if this model type isn’t yet a part of your research. Using the Cre-lox system in an experiment seems simple: two lines are crossed, one with tissue-specific
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Gaining Inducible Control

Inducible control of your tissue-specific animal model by Kristen Couglin The Cre-lox system is commonly used to provide tissue-specific gene knockout.  In order for this to happen in vivo, two mouse lines are mated together – The tissue-specific Cre line and a second mouse line containing your gene of interest flanked by loxP sites (floxed
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Mouse Model of Lymphoma

Taking control with a conditional point mutation model When designing a point mutation mouse model you should ask yourself, “Is a mouse with this mutation present in every cell the best model for my research?” It may make more sense to only express mutant sequence in specific cells, for example when making a cancer model.  There
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“iTL recently produced a new knockout model for us from a KOMP targeting vector (knockout first, conditional potential). The entire process was extremely efficient and the staff at iTL were both knowledgeable and helpful. We chose iTL because of their cost-effective service and their guarantee of germline transmission when using a KOMP targeting vector. Now that the project is complete, I would definitely use their services again in the future and would recommend them highly to anyone looking to create a new mouse model.”

-Morgan Fullerton, PhD