ingenious targeting laboratory has developed over 100 humanized p53 mouse targeting vectors, or p53 humanized mouse models, which includes both standard and conditional p53 mutations. Using the elegant p53 targeting system developed by ingenious, investigators studying the p53 gene and relevant protein pathways now have at their fingertips a suite of validated, cost effective products and services at their disposal. We offer a selection of pre- made vectors and targeted ES cell lines, using our own robust and validated ES cells. The flexibility of this system allows for customizable and regulatable mutations that fall between exons 4-9 of p53. Genetically engineered mouse models of human disease, such as the p53, can provide new insights into cancer.
1) The information regarding these targeting vectors, including the detailed design down to the base pairs, can be visualized online here.
2) p53 technology validation: Hanel W, Marchenko N, Xu S, Yu SX, Weng W, Moll U. 2013. Two hot spot mutant p53 mouse models display differential gain of function in tumorigenesis. Cell Death Differ 20(7): 898-909.