The Importance of Using Appropriate Mouse Stem Cells and Following Culture Protocols
Mouse stem cells are used on a regular basis in relation to a wide array of experiments and research studies. The use of stem cells has become even more widespread through the development of mouse embryonic cell lines than through any other cell type. Since the goals of stem cell research have to do with obtaining healthy replacements and repairs for cells and organs affected by disease or injury, finding and following the best culture procedures and protecting cells from being contaminated or losing their identity are some of the most important requirements to take into account.
Types of Stem Cells Generating Different Varieties of Mature Cells
Stem cells have the possibility to develop into a wide variety of mature somatic state, regardless of where they come from. Since this transformation can happen both in vitro and in vivo, the use of in vitro cultivated mouse cell lines to achieve specific types of cell growths can lead to significant discoveries and results that could impact the entire field of cell stem research, if properly performed. Keeping that in mind, we have to understand the four main forms of ES cells can take, depending on the types of mature cells we’d like to develop: ES cells that are able to develop into any type of cell, induced pluripotent stem (iPS) cells capable of being reprogrammed into a pluripotent state, cancer stem cells for studying tumorigenesis and adult cells like hematopoietic stem cells, which are more restricted in their differentiation.
Beginning Studies Featuring Mouse Embryonic Stem Cells
Some of the first mouse studies associated with stem cell research involved removing mouse blastocysts from the uterine horns of female mice and dissolving the zona pellucida in .5 ml of acid Tyrode’s solution to gain access to the cells that would be cultivated. Mouse embryonic fibroblast (MEF) were often seeded several hours before the harvesting of the stem cells, in order to be used for starting the stem cell line. While the procedures used today are similar, it is much more streamlined, and researchers can usually purchase the ES cells, instead of trying to obtain them from a mouse blastocyst.
Mouse Cell Lines and Their Impact on Cell Culture Studies
Let’s face it, you can take a long time to grow and develop actual mouse models and direct their genetic development just the way you want. Also, in many cases, you don’t need living mice to conduct your experiments, and using primary cells can be a more difficult and cumbersome process, especially when you want accuracy when it comes to the cell cultures and strains you ultimately want to get. Using readily available mouse cell lines for your research is an additional alternative that you might not have considered. Mouse ES cell lines can be purchased according to the specific requirements of your research and grown almost indefinitely.
The Importance of Proper Culture Procedure and Accurate ES Cell Line Identification
Proper culture procedure is essential when it comes to conducting safe and accurate mouse stem cell experiments. Authors of studies and researchers are also urged to be extremely careful about identifying and authenticating specific cell lines, as failure to do so could result in the voiding of all results. Misidentified cells, contaminated samples and inadequate culturing protocols can lead to inaccurate study results that can often lead to mistaken conclusions about the effect of specific stem cell and genetic therapy procedures.