From classic models to cutting edge technologies, ingenious has been advancing the field of genetically engineered mice for nearly two decades. In that time we have developed and acquired unique technologies that foster responsible innovation, that is, tools that improve the quality and speed of generating mouse models but without excess materials and animals. This expands capabilities with KO mice, KI mice, humanized and other variations. This sets ingenious apart, allowing you, the researcher, to create models that exceed expectations. Our team of industry veterans will continue to pursue new technologies that challenge conventional wisdom and stretch the possibilities of mouse model generation.
TruHumanization™: Improving Humanized Mouse Models.
ingenious offers TruHumanization™ as a solution to the challenges presented by standard methods to humanize the mouse. By utilizing large BACs, we are able to replace the mouse gene of interest with the entire human gene sequence including promoter, intron, exon, and regulatory sequences, in a single targeting step. This saves time and money, leads to more faithful gene regulation and processing, and allows the potential to perform functional and regulatory studies on multiple transcripts.
CRISPR Assisted Gene Targeting in ES Cells: Combining accuracy and timeliness while minding the 3 Rs.
We offer CRISPR-Assisted targeting which provides the advantage of screening cells instead of mice, while at the same time reducing timelines and costs. CRISPR-Assisted targeting provides the speed of CRISPR with the reliability of projects screened in ES cells.
BAC to BAC: Large Scale Targeting at ingenious: Base designs around experiments, not limitations.
At ingenious 200 kb is the new standard. Our mission is to push past size limits, leading to the development of proprietary BAC and fosmid technologies that reduce the time associated with these projects to a fraction of their typical imposing scale. Design your model around what is needed, not what someone says is possible.
Inducible Rosa26-Express™ Targeting
We have taken the complicated process of generating tissue-specific and inducible transgene expression systems and made it simple. Our technology utilizes a floxed stop cassette and all of the necessary Tet-On control components, enabling tissue-specificity and inducibility without the need for transactivator or silencer mouse lines. Simply mate the targeted transgenic line produced for you by ingenious with your tissue-specific Cre recombinase mouse line of choice and introduce doxycycline for inducible/reversible expression of your transgene in your tissue of interest.
No-Doubt Conditional Knockouts™: Certainty through GFP
Verifying that Cre recombinase has successfully disrupted your target gene can be challenging, particularly when working with tissue-specific Cre expression. ingenious’ No-Doubt Conditional KnockoutTM system makes the process simple. Utilizing our proprietary split-GFP design, reporter expression is tied to the recombination event and the restoration of GFP. Our technology removes concerns about leakiness and limited or low expression from the local gene promoter, and provides a reliable visual cue to show that your target gene has been knocked out.
ingenious’ Split Cre Technology: Overcome Cre mice development challenges.
Cre expressing mouse lines that were previously unavailable due to toxicity can now be generated without risk using ingenious’ Split Cre technology.
Rapid-Rosa26™ Targeting: Improving Targeted Transgenics. Rapid-Rosa26™ Targeting models are ingenious’ solution to the request for custom targeted transgenic models with shorter production timelines and reduced cost, without compromising performance and quality. By utilizing well-validated gene targeting strategies combined with our ready-to-go enhancing vectors, we deliver your expression cassette or cDNA to the Rosa26 locus in record time. This allows for the generation of precisely targeted, custom transgenic mouse models with a variety of expression possibilities at up to 25% reduced time and cost when compared to competitor technologies.
Inducible/Reversible Regulation of Gene Expression with F.A.S.T.™ Technology
Demonstrated in Science, our exclusively licensed F.A.S.T.™ (Flexible Accelerated STOP TetO) technology is a versatile tool where a single targeting event yields a multi-purpose mouse line with the potential for five or more controllable functionalities from the same locus, just using mating. The models that can be generated include: global gene knockout, tissue & time-specific gene rescue, ectopic gene expression, inducible & reversible gene over expression, and inducible & reversible gene knockdown.
FLP Embryonic Stem Cells: One Step Ahead of the Rest.
Our proprietary highly germline competent FLP ES cells alleviates the need for breeding out the Neo selection cassette, which in turn saves 3-6 months of mouse production time and cost.
Mouse Embryonic Stem Cell Lines: The Secret to ingenious’ Success.
Our success lies within our high-performing mouse embryonic stem cell lines. Tested regularly for quality and extensively validated, all of our ES cell lines have a greater than 90% euploidy rate, compared to the 70% industry standard. From our lines, over 1,650 gene targeted mouse models have been delivered. Hundreds of gene targeted models generated by ingenious have been published in high impact journals, including in Science, Nature, and Cell.
p53 Humanized Mouse Models
ingenious has developed over 100 humanized p53 mouse targeting vectors, including both standard and conditional p53 mutations. Using our elegant p53 targeting system, investigators studying the p53 gene and relevant protein pathways now have at their fingertips a suit of validated, cost effective products and services. The flexibility of our system allows for customizable and regulatable mutations that fall between exons 4-9 of p53.
ingenious’ Colony Management Prediction Tools
Managing mouse colonies can be time consuming and costly, especially in the absences of a proper colony management plan. By utilizing proprietary modeling and prediction software, we can provide the guidance and tools needed to optimize your colony. No matter where you are in the management of your mouse colony, we can help plot your course to success, making the management of your mouse lines easy and straight-forward.