BAC to BAC: Large Scale Gene Targeting
For too long, mouse model designs have been dictated by size constraints imposed by the limitations of technology. At ingenious, our mission to push past limits has led to the development of proprietary BAC and fosmid technologies that reduce the time associated with these projects to a fraction of their typical imposing scale.
From the initial project concept, to identifying positive clones, to confirming F1 neo deleted mice, all within a year- 200 kb targeting is the new “standard size” at ingenious targeting laboratory. Design your model, the center of your research, around what is needed to advance your field, not just what someone else tells you is possible.
Combining BAC targeting with our other technologies:
No-Doubt Conditional Knockout™: Certainty through GFP
Flank entire genes with ingenious’ next generation conditional knockout system in a single targeting step. By combining reliable, long range BAC targeting with ingenious’ split-GFP technology, verification of your BAC scale knockout has never been more clear.
Replace an entire genomic mouse gene with the human ortholog, including introns, in a single targeting step. Intronic sequences contain regulatory features, microRNA genes, promoters, enhancers, and splice sites for transcript isoforms. Ensure that you are working with the complete picture, because a gene is more than just the protein that it encodes.
Point Mutation Models
Introduce as many point mutations as needed in a single targeting step with a reliable timeline. Alternative approaches, such as CRISPR, require multiple independent targeting events to achieve the same result. The odds of multiple targeting events occurring on the same allele are quite low and decrease with each additional mutation required.
Delete entire genes and gene clusters in a single targeting step without the fear of truncated protein expression.
From co-expression to fusion, to reporter tagging and more- ingenious can achieve these designs in a single targeting step. Introduce the exogenous sequences that your research requires, not what is simply available utilizing outdated technologies. Learn more about our knockin mouse models.
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“Our experience with ingenious is a long one with several knock-in models engineered in the last seven years. Everytime the entire process was efficiently and successfully achieved even when challenged by unexpected difficulties. We are fully satisfied with their services and we would like to underline the quality of the ingenious professionals with a very special mention for project managers. We enthusiastically recommend iTL and we will request their services in the future. No doubts about it.”