BAC to BAC: Large Scale Targeting at ingenious

Large Scale Gene Targeting

At ingenious, 200kb is the new standard. No restrictions. Only possibilities.
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BAC To BAC: Large Scale Targeting

For too long, mouse model designs have been dictated by size constraints imposed by the limitations of technology. At ingenious, our mission to push past limits has led to the development of proprietary BAC and fosmid technologies that reduce the time associated with these projects to a fraction of their typical imposing scale.

From the initial project concept, to identifying positive clones, to confirming F1 neo deleted mice, all within a year- 200 kb targeting is the new “standard size” at ingenious targeting laboratory. Design your model, the center of your research, around what is needed to advance your field, not just what someone else tells you is possible.

Combining BAC targeting with our other technologies:

No-Doubt Conditional Knockout™: Certainty through GFP

Flank entire genes with ingenious’ next generation conditional knockout system in a single targeting step. By combining reliable, long range BAC targeting with ingenious’ split-GFP technology, verification of your BAC scale knockout has never been more clear.


Replace an entire genomic mouse gene with the human ortholog, including introns, in a single targeting step. Intronic sequences contain regulatory features, microRNA genes, promoters, enhancers, and splice sites for transcript isoforms. Ensure that you are working with the complete picture, because a gene is more than just the protein that it encodes.

Point Mutation Models

Introduce as many point mutations as needed in a single targeting step with a reliable timeline. Alternative approaches, such as CRISPR, require multiple independent targeting events to achieve the same result. The odds of multiple targeting events occurring on the same allele are quite low and decrease with each additional mutation required.

Knockout Models

Delete entire genes and gene clusters in a single targeting step without the fear of truncated protein expression. Learn more out a CRISPR knockout model.

Knockin Models

From co-expression to fusion, to reporter tagging and more- ingenious can achieve these designs in a single targeting step. Introduce the exogenous sequences that your research requires, not what is simply available utilizing outdated technologies. Learn more about our knockin mouse models and transgenic mice.

Cre-Lox Facts

6 Facts You May Not Know About Cre-Lox By Richard Row, PhD The basics of conditional knockout mice are familiar to any researcher who uses mouse models, even if this model type isn’t yet a part of your research. Using the Cre-lox system in an experiment seems simple: two lines are crossed, one with tissue-specific
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Gaining Inducible Control

By Kristen Coughlin Inducible Control of Your Tissue-Specific Animal Model The Cre-lox system is commonly used to provide tissue-specific gene knockout.  In order for this to happen in vivo, two mouse lines are mated together – The tissue-specific Cre line and a second mouse line containing your gene of interest flanked by loxP sites (floxed
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How Do I Start My Custom Model Project?

Begin your custom animal model in 3, 2, 1… Gene of interest? Check. Model type? Check. Species and strain? Check. Great, let’s get started! When you are ready to start a custom animal model project with us, we’re ready to move your project forward, too. Step 1: Sign your project quote and let your sales
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The Value Of Knockout Mouse Models

A Detailed Analysis of the Price and Value of Knockout Mouse Models According to a recent report on the pricing analysis of knockout mouse models, there are many more factors involved in determining the monetary value of the mice, aside from the importance of the research studies they are used in. Demand, availability, the complexity
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Knockout Mice In Cushing’s Syndrome Research

New Insight on the Effects of the ARMC5 Gene on Patients Suffering from Cushing’s Syndrome Genetic experiments and knockout mice have recently helped scientists at the University of Montreal Hospital Research Centre (CRCHUM) gain new insight on the effects that eliminating a specific gene known as ARMC5 can have in inducing particular ailments and predispositions
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Knockout Mouse In Circadian Rhythm Study

Scientists Discover a Faster and More Accurate Method of Studying the Circadian Rhythm Nearly all animals that follow a 24-hour cycle are subject to the famous circadian rhythms – the physiological shifts that regulate our biology at a cellular level. These inner biological clocks are affected by numerous genetic factors that have a strong impact
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Reversible Infantile Liver Failure

The Study of RILF Through a Genetically Modified Murine Mouse Model While studying RILF (reversible infantile liver failure), a mitochondrial disease that can cause severe unbalance in liver functions during infancy, scientists in Japan have found that they can explore the mechanisms involved with the progress of the condition through the use of a new
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Cardiac Health

Cardiac Knockout Mice Show Role of Gene in Fat Metabolism Scientists know that the heart predominantly uses lipids as fuel in order to function. However, recent research conducted by molecular biologist Dr. Weiqin Chen of Augusta University seems to reveal that both too many and too few of the lipid droplets required for normal heart
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“Our experience with ingenious is a long one with several knock-in models engineered in the last seven years. Everytime the entire process was efficiently and successfully achieved even when challenged by unexpected difficulties. We are fully satisfied with their services and we would like to underline the quality of the ingenious professionals with a very special mention for project managers. We enthusiastically recommend iTL and we will request their services in the future. No doubts about it.”

-Fernando Arenzana-Seisdedos