Knockout Mouse Models
Knockout mice carry targeted deletions that eliminate gene function, enabling loss of function studies across every therapeutic area. ingenious targeting laboratory offers multiple knockout strategies tailored to your experimental requirements.
Conventional Knockout
Conventional knockout models carry constitutive null alleles that eliminate gene function in all tissues from the earliest developmental stages. These models provide straightforward interpretation of genes that are not required for embryonic viability.
Learn MoreConditional Knockout
Conditional knockout models use the Cre lox system to enable tissue specific or temporally controlled gene deletion. The floxed allele design preserves normal gene function until Cre recombinase excises a critical region, providing experimental control over when and where knockout occurs.
Learn MoreTissue Specific Knockout
By crossing floxed alleles with tissue specific Cre driver lines, researchers can study gene function in specific cell populations while maintaining normal function elsewhere. This approach is essential when conventional knockout causes embryonic lethality or systemic effects that obscure tissue specific phenotypes.
Learn MoreInducible Knockout
Inducible systems such as tamoxifen activated CreERT2 allow temporal control over gene deletion. This enables study of gene function in adult animals and avoids developmental compensation that can mask phenotypes in constitutive models.
Learn MoreKnockin Mouse Models
Knockin mice carry precise sequence insertions at defined genomic locations. Unlike random transgenesis, knockin targeting ensures predictable expression levels and eliminates position effects.
Point Mutation Knockin
Point mutation models introduce specific nucleotide changes to study disease associated variants, alter protein function, or modify regulatory elements.
Learn MoreReporter Knockin
Reporter knockin models express fluorescent proteins, enzymatic markers, or other reporters under control of endogenous regulatory elements. This enables visualization of gene expression patterns, lineage tracing, and cell isolation based on marker expression.
Learn MoreTag Knockin
Tag knockin models add epitope tags such as FLAG, HA, or V5 to endogenous proteins. These tags enable protein detection, purification, and interaction studies without requiring gene specific antibodies.
Learn MorecDNA Knockin
cDNA knockin replaces a gene with a coding sequence, often to express modified proteins, isoform variants, or humanized sequences. The targeted approach ensures expression under endogenous regulatory control.
Learn MoreHumanized Mouse Models
Humanized mice carry human gene sequences in place of mouse orthologs, enabling preclinical testing of human specific therapeutics and study of human disease mechanisms.
Gene Replacement Humanization
Complete gene replacement substitutes the entire mouse gene with its human ortholog, including regulatory elements that control expression. This approach preserves physiological expression patterns while providing human target sequences.
Learn MoreImmune Checkpoint Humanization
Humanized immune checkpoint models express human PD1, PDL1, CTLA4, LAG3, TIM3, or other checkpoint proteins. These models enable testing of checkpoint inhibitor antibodies in immunocompetent mice with functional immune systems.
Learn MoreReceptor and Target Humanization
Therapeutic antibodies often show species specificity that prevents testing in standard mouse models. Humanizing the target receptor or protein enables preclinical efficacy studies in physiologically relevant contexts.
Learn MoreBAC Transgenesis
Bacterial artificial chromosome (BAC) targeting enables integration of large genomic segments including complete genes with native regulatory elements. This approach is valuable when physiological expression patterns are essential.
Learn MoreThe ingenious targeting laboratory Approach
Scientific Consultation
Every custom mouse model project begins with scientific consultation. Our team reviews your research goals, evaluates targeting strategy options, and recommends the approach most likely to deliver the experimental capabilities you need.
Comprehensive Documentation
Every project includes detailed documentation of targeting design, germline transmission records, and genotyping protocols. This documentation supports ongoing research and future breeding programs.
What Researchers Say
“We are actively engaged in the production of a number of conditional mouse models with iTL. This collaboration has been simple on my end, just providing the gene accession numbers of each gene, and iTL recommending the strategies for each gene. The full range of mouse knockout services matches my needs well. I find iTL's service uniquely useful for my situation of needing different models for my research in a quick and efficient manner.”
— Mehboob Hussain, MD
University of Michigan Health
Selected Publications
Custom mouse models from ingenious targeting laboratory have contributed to over 800 peer reviewed publications.
Start Your Project
Ready to discuss your custom mouse model requirements? Our scientific team provides complimentary project consultation to help you design the optimal model for your research.
Custom Mouse Model Insights
Subscribe to Lab Signals for biweekly guides on mouse model design, targeting strategies, and research best practices from our PhD scientists.