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F.A.S.T. Technology Mice

F.A.S.T.™ (Flexible Accelerated STOP Tetracycline Operator) technology provides versatile inducible and reversible gene expression control, enabling multiple experimental approaches from a single knockin allele. ingenious targeting laboratory incorporated F.A.S.T.™ technology into custom mouse models for neuroscience, immunology, and metabolic disease research.

The F.A.S.T.™ system achieves a spectrum of controllable expression levels, streamlining mouse model generation by enabling knockout first, inducible expression, and conditional knockdown modes from one targeted allele. This flexibility maximizes research value while minimizing project timelines and costs.

2,500+
Projects Completed
800+
Publications
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Years Experience
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Success Rate
✦ New for 2026

Breeding Scheme Architect

Plan complex single allele breeding strategies, calculate expected genotype ratios, and estimate time to experimental cohorts—all before starting your project.

Visualize breeding paths
Calculate Mendelian ratios automatically
Estimate timeline to study ready cohorts

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Allele 1Gene-flox (conditional)
Allele 2Cre-driver (tissue-specific)
TargetHomozygous knockout

→ 3 generations to target genotype

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Our scientific consultants are ready to discuss your research requirements and recommend the optimal approach for your program. Initial consultation is provided at no charge.

Frequently asked questions

F.A.S.T. (Flexible Accelerated Speed Targeting) is a gene targeting technology that enables rapid generation of genetically engineered mouse models. The technology uses optimized targeting vectors and streamlined ES cell targeting workflows to reduce timelines while maintaining high-quality targeting and pre-germline characterization.

F.A.S.T. technology can be used for knockout models, conditional knockout models, knockin models (point mutations, reporters, tags), and humanized models. The technology is compatible with standard allele architectures including knockout-first (tm1a) and derivative allele systems

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F.A.S.T. Technology Insights

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