Conditional Gene Targeting Systems
Cre Lox System
Conditional gene modification by placing critical exons between LoxP recognition sites. Cre expression catalyzes recombination to delete flanked sequences.
- •Tissue specific gene deletion through Cre driver lines
- •Temporal control via inducible Cre systems
- •Flexible allele design with multiple derivative options
- •Widely used and well characterized system
FLP FRT System
Orthogonal recombination approach working independently or with Cre lox, primarily for selection cassette removal and complex multi recombinase strategies.
- •Independent of Cre lox system
- •Selection cassette removal
- •Enables derivative allele generation
- •Supports dual recombinase approaches
Dre Rox System
Third orthogonal recombinase system enabling sophisticated intersectional strategies requiring expression of multiple recombinases.
- •Orthogonal to both Cre and FLP
- •Intersectional genetic strategies
- •Complex conditional control
- •Multi lineage experiments
Inducible Expression Systems
Tamoxifen Inducible (CreERT2)
Temporal control over gene deletion. CreERT2 remains inactive until tamoxifen administration enables gene deletion at defined timepoints.
- Permanent genetic change after single administration
- Wide variety of tissue specific CreERT2 lines available
- Well characterized and reliable system
- Enables adult deletion of developmentally essential genes
F.A.S.T.™ Technology
Flexible Accelerated STOP Tetracycline Operator provides versatile inducible/reversible control enabling multiple expression modes from single knockin allele.
- Knockout first mode
- Inducible expression mode
- Conditional knockdown/knockout mode
- Single allele multiple applications
Proprietary Technologies
Rapid Rosa26™ Targeting
Accelerates transgenic model generation through established protocols and proven vector backbones, enabling shorter timelines with reliable, high quality models.
Learn moreTruView Conditional Knockout™
Guarantees strong reporter expression after knockout, enabling reliable visualization of recombined cells regardless of target gene expression levels.
Learn moreTruHumanization™
Optimizes humanization strategies by analyzing human mouse protein homology, functional domain conservation, and therapeutic target requirements.
Learn moreAdvanced Targeting Strategies
Conditional Targeting Systems
Generation of multiple model types using Cre-LoxP and FLP-FRT systems, enabling flexible conditional and constitutive knockout approaches.
Learn moreBAC to BAC Large Scale Targeting
Targeting of large genomic regions up to 200kb, enabling complex humanization and multi gene targeting projects.
Learn moreSafe Harbor Locus Targeting
Safe harbor loci including Rosa26, HPRT, and H11 provide predictable transgene integration with stable expression and minimal positional effects.
Learn moreWhat Researchers Say
“I've been working with iTL over the past 5 years in the production of 3 different genetically altered mice. Not only did iTL help in the design of the mice, but the entire process was transparent with the opportunity at any time along the way to discuss my questions or concerns with scientists who had significant insight into the process. The mice were delivered on time, as billed!”
— Raghu Mirmira, MD, PhD
University of Chicago
Start Your Technology Consultation
Our scientific consultants can help you select the optimal technologies for your research goals. From basic gene targeting through advanced conditional and inducible systems, we guide technology selection to match your experimental requirements.