ingenious targeting laboratory offers targeted transgenic approaches using ROSA26 and other safe harbor loci, providing options matched to your experimental requirements.
Transgenic Approaches
Conventional Transgenesis
Conventional transgenic mice are generated by pronuclear injection of DNA constructs into fertilized oocytes. The transgene integrates randomly into the genome, typically as a multi copy array. This approach is rapid and cost effective for applications where precise copy number and integration site are not critical.
Commonly used for overexpression studies, generation of multiple founder lines for comparison, and applications where high expression levels are desired.
BAC Transgenesis
BAC (bacterial artificial chromosome) transgenic mice carry large genomic fragments up to 200 kb, including the gene of interest with its native regulatory elements. BAC transgenics often show more physiological expression patterns than conventional transgenics because distant enhancers and regulatory regions are preserved.
Particularly valuable for generating Cre driver lines where accurate tissue specific expression is essential.
ROSA26 Targeted Transgenics
ROSA26 targeted transgenics insert transgene constructs at the ROSA26 safe harbor locus. This approach provides single copy integration at a defined genomic location with predictable expression characteristics.
Eliminates the positional effects common with random integration and ensures consistent expression across breeding generations.
Learn more about ROSA26Other Safe Harbor Loci
In addition to ROSA26, transgenes can be targeted to other safe harbor loci including HPRT and H11. Each locus offers distinct expression characteristics and can be selected based on desired expression levels and tissue distribution.
Applications
Cre Driver Lines
Transgenic Cre driver lines express Cre recombinase under tissue specific promoters, enabling conditional gene manipulation when crossed to floxed alleles. At Ingenious, Cre drivers can be targeted to the ROSA26 locus or to an endogenous gene of interest.
Learn moreReporter Lines
Transgenic reporter lines express visualization markers under constitutive or tissue specific promoters. Common reporters include fluorescent proteins for live imaging, luciferase for bioluminescence studies, and LacZ for histological analysis. ROSA26 targeted reporters provide consistent expression and can be designed as Cre dependent systems for lineage tracing applications.
Inducible Expression Systems
Transgenic mice carrying inducible expression systems allow controlled activation of transgene expression.
Inducible transgenics enable temporal control over gene expression for studying acute effects and adult onset phenotypes.
Technical Considerations
Targeted Integration Advantages
ROSA26 and other targeted approaches provide:
Selected Publications
Rosa26 models generated by ingenious targeting laboratory:
Serrano J, Boyd J, Brown IS, Mason C, Smith KR, Karolyi K, Maurya SK, Meshram NN, Serna V, Link GM, Gardell SJ, Kyriazis GA. (2024).
The TAS1R2 G-protein-coupled receptor is an ambient glucose sensor in skeletal muscle that regulates NAD homeostasis and mitochondrial capacity ↗Nat Commun 15(1): 4915
What Researchers Say
“We are very happy with this mouse line and we're not done yet. There's still plenty to do. The versatility of the F.A.S.T.™ approach is really unparalleled. There are so many possibilities to use the cassette that one paper does not showcase it all. Also, the addition of N-terminal GFP has helped quite a bit to understand gene expression patterns.”
— Frank Bosmans, PhD
Ghent University, Belgium
Start Your Transgenic Project
Our scientific consultants are ready to discuss your research requirements and optimal targeted transgenic approach for your experimental goals. Initial consultation is provided at no charge and includes construct design review, approach recommendations, timeline, and price estimate.
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