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Safe Harbor Locus Integration Strategies

Since 1998, ingenious targeting laboratory has employed safe harbor locus targeting in over 800 custom mouse models, enabling predictable transgene expression with minimal disruption to endogenous mouse genomics and contributing to 215+ peer reviewed publications using safe harbor integration approaches. Safe harbor loci are genomic locations that tolerate large DNA insertions without disrupting essential genes, altering developmental patterns, or creating unpredictable expression effects.

Unlike random integration or multi-copy insertions, safe harbor targeting provides consistent, copy number controlled expression while preserving normal mouse biology. This approach has become essential for researchers requiring reliable transgene expression combined with genetic predictability.

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Breeding Scheme Architect

Plan complex multi-allele breeding strategies, calculate expected genotype ratios, and estimate time to experimental cohorts—all before starting your project.

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Allele 1Gene-flox (conditional)
Allele 2Cre-driver (tissue-specific)
TargetHomozygous knockout

→ 3 generations to target genotype

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Our scientific consultants are ready to discuss your research requirements and recommend the optimal approach for your program. Initial consultation is provided at no charge.

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Frequently asked questions

Safe-harbor loci are genomic locations that support transgene integration without disrupting endogenous gene function or causing position effects. Rosa26, H11, and HPRT are well-characterized safe-harbor loci that provide predictable, stable transgene expression with minimal variability between founder lines.

Safe-harbor integration provides single-copy integration at a defined location, predictable expression patterns, minimal position effects, and reproducible results across founder lines. Random integration can cause variable copy numbers, position effects, disruption of endogenous genes, and inconsistent expression.

Rosa26 is the most widely used safe-harbor locus, providing ubiquitous expression suitable for most applications. H11 provides an alternative with similar properties. HPRT is used for specific applications. Selection depends on expression requirements, construct size, and experimental goals.

Yes. Safe-harbor loci can accommodate conditional expression cassettes including LoxP-stop-LoxP for Cre-dependent expression, TRE-driven systems for doxycycline control, and other inducible systems. The safe-harbor provides the integration site; expression control depends on construct design. (/request-quote) Last Updated: December 23, 2025

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