Custom Rat Models
Our advanced process enables genome editing directly within the rat’s germline. This allows for consistent and precise targeting, removes concerns and extra breeding due to mosaicism, and achieves germline transmission faster than traditional approaches.
We target spermatogonial stem cells to create our rat custom models, a method described in this paper published in Neuron. With this technology, ingenious is able to create similar models for your research needs.
- More predictive power
- Mosaicism eliminated
- Enhanced speed
Get your custom rat model in less time & with more certainty.
What Types of CRISPR Rat Models Are Possible?
The types of custom rat models that we can make for you are rat knockins that include a point mutation knockin, a knockin with reporter, an inducible/reversible knockin, and a Rosa26 rat knockin. Rat knockout models include a constitutive knockout and a conditional knockout.
Rat Point Mutation Knockin
Rat Knockin w/ Reporter
Constitutive Rat Knockout
Rosa26 Rat Knockin
Rat Inducible/Reversible KI
Conditional Rat Knockout
Rat Strains Available
Gene targeting in diverse rat strains, including:
- Why Rat Models?
Rats have been used to model human disease for over a century within a broad range of science fields including neurobiology, cardiology, immunology and toxicology. Their size and physiological similarity to humans make them an ideal choice for many labs. For example, surgical manipulations can be performed on rats that are difficult or impossible with smaller rodents, and fewer animals are required to generate sufficient material for experiments. Previously it was difficult to genetically modify rats but these limitations have been overcome by the use of CRISPR technology. Coupling CRISPR with our sperm stem cell technology takes this one step further, alleviating issues of consistency, predictability and mosaicism which are common when using CRISPR in embryos.
1) Bäck S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK. 2019. Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Neuron 102(1):105-119.e8.
2) Chapman KM, Medrano GA, Jaichander P, Chaudhary J, Waits AE, Nobrega MA, Hotaling JM, Ober C, Hamra FK. 2015. Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells. Cell Rep 10(11): 1828-35.