CRISPR Rat Models
ingenious offers custom genetically modified rats, an exciting new option for your next genetically modified rodent model. Our unique technology delivers CRISPR rat models with unparallelled speed and accuracy by making targeted genetic modifications in spermatogonial stem cells. Placing modified cells into host rat guarantees germline transmission to rapidly establish your new line. This robust system provides a cost effective, scalable platform for the production of rats with enormous predictive power that will shape development of better therapies, pushing drug discovery research into a new era.
Pushing the Evolution of Rat Model Technology
More Predictive Power
Germline In 3 Months
Enhanced Predictability & Speed of CRISPR Rat Models
This advanced process enables genome editing directly within the rat’s germline, using CRISPR-Cas9 to make targeted modifications in spermatogonial stem cells. The portfolio of patent-pending sperm stem cell gene editing technologies fill key efficiency gaps in the production of genetically modified model organisms. This allows for consistent and precise targeting, removes concerns and extra breeding due to mosaicism, and achieves germline transmission in three months versus six with traditional technologies.
COMPARE TIMELINES FOR RAT MODEL PRODUCTION:
Why Rat Models?
Rats have been used to model human disease for over a century within a broad range of science fields including neurobiology, cardiology, immunology and toxicology. Their size and physiological similarity to humans make them an ideal choice for many labs. For example, surgical manipulations can be performed on rats that are difficult or impossible with smaller rodents, and fewer animals are required to generate sufficient material for experiments. Previously it was difficult to genetically modify rats but these limitations have been overcome by the use of CRISPR technology. Coupling CRISPR with our sperm stem cell technology takes this one step further, alleviating issues of consistency, predictability and mosaicism which are common when using CRISPR in embryos.
What Types of Rat Models Are Possible?
The types of Custom Rat Models that are possible to produce are rat knockins that include a point mutation knockin, a knockin with reporter, an inducible/reversible knockin, and a Rosa26 rat knockin. Rat knockout models include a constitutive knockout and a conditional knockout.
Rat Point Mutation Knockin
Rosa26 Rat Knockin
Rat Knockin w/Reporter
Rat Inducible/Reversible KI
Constitutive Rat Knockout
Conditional Rat Knockout
Rat Strains Available
Gene targeting in diverse rat strains, including:
Chapman KM, Medrano GA, Jaichander P, Chaudhary J, Waits AE, Nobrega MA, Hotaling JM, Ober C, Hamra FK. 2015. Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells. Cell Rep. 10:1828-35