CRISPR Rat Models

Custom Rat Models

In just three months. Mosaicism eliminated.
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ingenious offers custom genetically modified rats, an exciting new option for your next genetically modified rodent model. Our unique technology delivers CRISPR rat models with unparallelled speed and accuracy by making targeted genetic modifications in spermatogonial stem cells. Placing modified cells into host rat guarantees germline transmission to rapidly establish your new line. This robust system provides a cost effective, scalable platform for the production of rats with enormous predictive power that will shape development of better therapies, pushing drug discovery research into a new era.

Get your custom rat model- in less time & with more certainty.

More Predictive Power

Mosaicism Eliminated

Germline In 3 Months

Enhanced Predictability & Speed of CRISPR Rat Models

Our advanced process enables genome editing directly within the rat’s germline. This allows for consistent and precise targeting, removes concerns and extra breeding due to mosaicism, and achieves germline transmission in three months versus six with traditional approaches.

What Types of Rat Models Are Possible?

The types of Custom Rat Models that are possible to produce are rat knockins that include a point mutation knockin, a knockin with reporter, an inducible/reversible knockin, and a Rosa26 rat knockin. Rat knockout models include a constitutive knockout and a conditional knockout.


Rat Point Mutation Knockin

Rosa26 Rat Knockin

Rat Knockin w/Reporter

Rat Inducible/Reversible KI

Constitutive Rat Knockout

Conditional Rat Knockout

Transgenic Rat

Transgenic knockin rat models are powerful tools for research with many applications. Express one or more sequences throughout the animal or only in specific tissues. Common strategies include expression of mutated gene sequences, human gene sequences, and creation of tissue-specific reporter lines.

Rat Knockout

A custom knockout rat model from ingenious will support the research goals of your lab. A knockout line can model human disease, help understand a newly-discovered gene, and for drug discovery and development.

Rat Strains Available

Gene targeting in diverse rat strains, including:

Why Rat Models?

Rats have been used to model human disease for over a century within a broad range of science fields including neurobiology, cardiology, immunology and toxicology. Their size and physiological similarity to humans make them an ideal choice for many labs. For example, surgical manipulations can be performed on rats that are difficult or impossible with smaller rodents, and fewer animals are required to generate sufficient material for experiments. Previously it was difficult to genetically modify rats but these limitations have been overcome by the use of CRISPR technology. Coupling CRISPR with our sperm stem cell technology takes this one step further, alleviating issues of consistency, predictability and mosaicism which are common when using CRISPR in embryos.


Chapman KM, Medrano GA, Jaichander P, Chaudhary J, Waits AENobrega MA, Hotaling JMOber C, Hamra FK. 2015. Targeted Germline Modifications in Rats Using CRISPR/Cas9 and Spermatogonial Stem Cells. Cell Rep. 10:1828-35

Understanding Rat Models Of Various Human Disease

Rat Models of Human Disease The history of using rats to study human disease goes back over 150 years, having been a favorite laboratory research animal, and we are all familiar with the term “lab rat.” While the use of mice in research has grown significantly over the past few decades, that increase being driven by

Cardiovascular Disease

By Richard Row, Ph.D. Rat Models of Cardiac Disease Cardiovascular disease is the most frequent cause of death worldwide at over 17 million deaths per year – over 30% of all deaths. Coronary heart disease alone kills more than 7 million people every year. Where available, proactive health care measures such as risk factor screening,

The Advantages Of Rat Models

By Kristen Coughlin The Rat as a Model System Rats were the first mammalian species specifically domesticated to be used in the laboratory. Two centuries ago, the brown rat Rattus norvegicus was used by scientists to understand human physiology and medicine, concentrating on the effects of food and oxygen deprivation. Rats have since been used


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“We found iTL’s service to be exceptionally efficient and very well managed. We generated several mouse lines with iTL, and every step for each project was performed quickly and precisely, without any delays or problems.”

-Santos Franco, PhD