Inducible / Reversible (F.A.S.T.™)

F.A.S.T Technology

Exclusively by ingenious.

Contact us

Inducible/Reversible F.A.S.T.™

Demonstrated in Science [1], our exclusively licensed F.A.S.T.™ (Flexible Accelerated STOP TetO) technology is a versatile tool where a single targeting event yields a multi-purpose mouse line with the potential for five or more controllable functionalities from the same locus, just using mating. [2]

These functionalities can include:

  • Global gene knockout

  • Tissue/time-specific gene rescue

  • Ectopic gene expression

  • Inducible/reversible gene overexpression

  • Inducible/reversible gene knockdown

 

Depicted in the schematic below, Tanaka KF et al showed that the F.A.S.T.™ system achieves a spectrum of controllable expression levels from the Mlc1 gene, thereby streamlining the mouse model generation process.

F.A.S.T.™
Flexible Accelerated STOP Tetracycline Operator

FAST Schematic from GeneTargeting.com

 

As a knockout first, the Mlc1 gene function was rescued by mating the initial mouse line to a tissue specific Cre line of choice. Alternatively, gene expression can be induced through the F.A.S.T.™ cassette. A tetracycline transactivator (tTA) line was used to produce an ectopic gene expression model, and a tissue-specific over-expression model. A tetracycline trans-silencer line was used to generate a tissue-specific conditional knockdown/knockout.

These principals are possible for your gene of interest using the F.A.S.T.TM cassette, making your research more flexible.


Other Resources

Whitepaper – The F.A.S.T. (TM) Gene Modulating System

Tet Systems: principles and components

Tet Systems: homepage

Schönig K et al. (2010) The power of reversibility: regulating gene activities via tetracycline-controlled transcription. Methods Enzymol. 477: 429-453.

Stieger K et al. (2009) In vivo gene regulation using tetracycline-regulatable systems. Adv Drug Deliv Rev 61(7-8): 527-541. Review.



CONTACT US

Fields marked with an * are required.