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F.A.S.T.™ Technology

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Inducible/Reversible F.A.S.T.™ Technology, exclusively by ingenious.

Demonstrated in Science¹, our exclusively licensed F.A.S.T.™ (Flexible Accelerated STOP TetO) technology is a versatile tool where a single targeting event yields a multi-purpose mouse line with the potential for five or more controllable functionalities from the same locus, just using mating.² These functionalities can include global gene knockout, tissue/time-specific gene rescue, ectopic gene expression, inducible/reversible gene overexpression, and inducible/reversible gene knockdown.

F.A.S.T.™

Flexible Accelerated STOP Tetracycline Operator

Depicted in the schematic below, Tanaka KF et al showed that the F.A.S.T.™ system achieves a spectrum of controllable expression levels from the Mlc1 gene, thereby streamlining the mouse model generation process.

As a knockout first, the Mlc1 gene function was rescued by mating the initial mouse line to a tissue specific Cre line of choice. Alternatively, gene expression can be induced through the F.A.S.T.™ cassette. A tetracycline transactivator (tTA) line was used to produce an ectopic gene expression model, and a tissue-specific over-expression model. A tetracycline trans-silencer line was used to generate a tissue-specific conditional knockdown/knockout.

These principles are possible for your gene of interest using the F.A.S.T.™ cassette, making your research more flexible.