What is F.A.S.T. technology and how does it work?

F.A.S.T. (Flexible Accelerated Speed Targeting) is a gene targeting technology that enables rapid generation of genetically engineered mouse models. The technology uses optimized targeting vectors and streamlined ES cell targeting workflows to reduce timelines while maintaining high-quality targeting and pre-germline characterization.

What types of models can be generated using F.A.S.T. technology?

F.A.S.T. technology can be used for knockout models, conditional knockout models, knockin models (point mutations, reporters, tags), and humanized models. The technology is compatible with standard allele architectures including knockout-first (tm1a) and derivative allele systems

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F.A.S.T. Technology Mice

F.A.S.T.™ (Flexible Accelerated STOP Tetracycline Operator) technology provides versatile inducible and reversible gene expression control, enabling multiple experimental approaches from a single knockin allele. ingenious targeting laboratory incorporated F.A.S.T.™ technology into mouse model generation for neuroscience, immunology, and metabolic disease research.

The F.A.S.T.™ system achieves a spectrum of controllable expression levels, streamlining mouse model generation by enabling knockout first, inducible expression, and conditional knockdown modes from one targeted allele. This flexibility maximizes research value while minimizing project timelines and costs.

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