Inducible Rosa26-Express™ Targeting

achieve inducible conrol of your mouse

At ingenious targeting laboratory, we utilize precision gene targeting to generate your transgenic mouse model. Our proprietary technologies allow for shorter production timelines and reduced costs when compared to other targeting methods, without compromising performance and quality.  We efficiently introduce your overexpression construct into the genome in a location where it will not interfere with other genes or be affected by the surrounding genetic environment, for more predictable results.

Various options are available for the customized design of your transgenic mouse model, depending on your unique needs, including knockin to specific genes, safe-harbor transgenic knockin, conditional overexpression, and drug-inducible systems. Our models are versatile tools for studying human disease, drug testing, and gene function analysis.

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“iTL has done a tremendous job assisting us with our projects. Not only have they provided successful mouse lines, but their project management has always been on top of things. Communication was excellent, and at all times I felt I could trust the scientists at iTL with my work.”– Thomas G.H. Diekwisch, DMD, PhD (sc), PhD (phil.) Texas A&M University College of Dentistry

Inducible Rosa26-Express™ Targeting

Introducing ingenious’ Inducible Rosa26-Express™ Targeting

ingenious has taken the complicated process of generating tissue-specific inducible transgene expression systems and simplified it. By designing a targeting vector that contains a floxed stop cassette and all of the necessary Tet-On control components, we enable tissue-specificity and inducibility without the need for transactivator or silencer mouse lines. Simply mate the targeted transgenic line with a tissue-specific Cre recombinase mouse line of choice and introduce doxycycline for inducible/reversible expression of your transgene in your tissue of interest.

Inducible Rosa26-Express™ Targeting Benefits:

Improve Inducible and Tissue-Specific Transgene Control from the Safe-Harbor Rosa26 Locus: ingenious’ Inducible Rosa26-Express™ system allows for easier inducible and tissue-specific control of transgenes.

Reduced Time and Cost: Our Inducible Rosa26-Express™ is combined with our Rapid-Rosa26™ Targeting technology to reduce the time and cost required to generate your mouse model.

Tissue Specificity via Cre Recombinase: Tissue specificity is determined by Cre recombinase expression. With a vast array of Cre expressing mouse lines readily available, finding the expression profile you need is easy and reliable.

Control Transgene Expression: Doxycycline provides inducible control over your transgene and allows for expression at specific ages, or for defined durations to meet experimental requirement.

Avoid Undesirable Embryonic or Developmental Phenotypes: Embryonic and/or developmental phenotypes can be a risk when introducing transgenes, especially if the transgenes are novel or not well characterized. Bypass these issues by preventing expression during early developmental stages.

How ingenious’ Inducible Rosa26-Express™ Targeting System Works

A graph depicting inducible cDNA expression using Doxycycline.

The design works by incorporating a reverse tetracycline-controlled transactivator (rtTA) component, a TRE3G (tetO) promoter, and a floxed Neomycin-Stop cassette into the “safe harbor” Rosa26 locus. The Neomycin-Stop cassette, inserted downstream of the CAG promoter, prevents rtTA expression, which is one of the key components required for transgene expression from the TRE3G promoter.