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Cre-Lox Facts

Cre-Lox Facts

6 Facts You May Not Know About Cre-Lox By Richard Row, PhD The basics of conditional knockout mice are familiar to any researcher who uses mouse models, even if this model type isn’t yet a part of your research. Using the Cre-lox system in an experiment seems simple: two lines are crossed, one with tissue-specific expression of Cre and one with
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Gaining Inducible Control

Gaining Inducible Control

Inducible control of your tissue-specific animal model by Kristen Couglin The Cre-lox system is commonly used to provide tissue-specific gene knockout.  In order for this to happen in vivo, two mouse lines are mated together – The tissue-specific Cre line and a second mouse line containing your gene of interest flanked by loxP sites (floxed mouse).  Cre recombines the loxP sites,
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Gene Knockin Mouse

Gene Knockin Mouse

Aha! I need a knockin mouse… Now what? by Jennifer Parla, PhD Moving to animal studies At the start of your research, a live animal model may not have been necessary. You may have been studying a biochemical pathway or process that is highly conserved and can be investigated using cultured cells or sometimes with cell-free assays. Or your research may
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Mouse Model of Lymphoma

Mouse Model of Lymphoma

Taking control with a conditional point mutation model When designing a point mutation mouse model you should ask yourself, “Is a mouse with this mutation present in every cell the best model for my research?” It may make more sense to only express mutant sequence in specific cells, for example when making a cancer model.  There is the possibility of addressing these
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Expand the Possibilities of Humanized Models

Expand the Possibilities of Humanized Models

by Richard Row, PhD …Beyond the Coding Sequence.   Human genetic sequences were knocked into the mouse genome almost as soon as reliable transgenesis techniques became available. These genetically humanized mice were quickly recognized as a means to study human diseases or produce specific valuable human proteins. Recent method development has expanded the possibilities for genetic humanization and enables the creation of
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A Conditional Knockout That Took Research Further

A Conditional Knockout That Took Research Further

The right myopathy model After years of work in in vitro systems and cultured cells your research may reach a point where an animal model is required. This major transition requires a new mindset, and consideration of factors that didn’t previously affect experiments. When evaluating genetically modified animal models each stage of their life must be considered. For example, it’s possible
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