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Build A Better Mouse Model

Build a Better Mouse Model, with 2A Peptide Strategies Researchers often ask us about ways to track a knockout or knockin allele in a new mouse model, or how to express multiple proteins in a single mouse model. Utilizing the 2A peptide is often an ideal solution. The 2A peptide system Several 2A peptide sequences are commonly used, each consisting of about 20 residues, which can serve as a linker between two different proteins. During translation a break is created in the protein product resulting in two proteins being produced from a single bicistronic mRNA. This co-expression paradigm enables creation of more versatile mouse models. With the 2A system Cre
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Eye Microbiome Trigger Immune Response in Mice Models

Study Proves a Resident Ocular Microbiome   “Bugs in your eyes may be a good thing.” New research is showing that immune responses that protect the eye from infection are due to microbes living on the surface of the eye. Research conducted at the National Eye Institute (NEI), part of the National Institutes of Health, indicates the existence of a resident ocular microbiome that trains the developing immune system to fend off pathogens. Until recently, it was thought that the surface the eye was sterile due to  the existence of an enzyme called lysozyme that eliminates bacteria, antimicrobial peptides, and other components that rid the eye of microbes that may
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Fixing Off Target Effects of CRISPR/Cas9

Fixing Off Target Effects of CRISPR/Cas9 CRISPRs simplicity and accessibility has given itself the oxygen to spread like wildfire through the research community, but so have it’s off target effects. Recently, scientists have used it in clinical trials for slowing or stopping the production of lung cancer cells, but due to these concerns about its off target effects, scientists are wondering how effective it will actually be in humans. This leads to another major question and concern, what the off target effects will be in human trials. Potential/Questionable Mutant Conclusions It was determined by Kellie A. Schaefer and collaborators at Stanford that the accuracy of CRISPR/Cas9 in regard to its
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A New Humanized Mouse Model

A New Humanized Mouse Model Leads to New Opportunities in Treating Colorectal Cancer While immunotherapy is effective in the case of many different types of cancer, colorectal cancers have always presented scientists with far greater challenges, due to being “microsatellite stable.” According to Julie Lang, PhD, the CU Cancer Center’s senior research associate, a recently developed humanized mouse model of colorectal cancer has allowed researchers to make significant progress in the application of immunotherapy for this type of cancer. The study was presented at the American Association for Cancer Research (AACR) 2017 Meeting, and it is the first to present a humanized mouse with a human-like immune system that has
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