Biomarker Discovery Mice

Common reporter knockins include fluorescent proteins (GFP, mCherry), luciferase for bioluminescence imaging, and epitope tags (HA, FLAG) for protein detection. Reporters can be knocked into endogenous loci to track gene expression or fused to proteins of interest for localization studies.

Yes. Inducible Cre (CreER) systems enable temporal control of reporter expression. TRE-based systems (tetracycline-responsive) provide reversible control. These systems enable tracking of biomarker expression at specific disease timepoints or in response to treatments.

Pre-germline characterization includes Southern blot analysis to confirm correct targeting and single-copy integration. Flow cytometry, immunohistochemistry, or Western blot confirms reporter expression and proper localization. Functional validation ensures reporter faithfully reflects endogenous gene expression.

Yes. Reporter knockins can be combined with disease models (knockouts, point mutations) to track biomarker expression in disease context. For example, reporter knockin combined with conditional knockout enables tracking of biomarker-expressing cells during disease progression or treatment response.