Site Specific Recombination
FLP FRT System
Since 1998, ingenious targeting laboratory has utilized the FLP FRT recombination system in hundreds of gene targeting projects, providing researchers with versatile tools for conditional gene expression and sophisticated allele conversion strategies.
The FLP FRT system offers an orthogonal recombination platform that complements Cre lox technology, enabling complex genetic manipulations including dual recombinase strategies and sequential allele conversion.
2,800+
Models Generated
800+
Publications
26+
Years Experience
900+
Laboratories Served
Applications of the FLP FRT System
Dual Recombinase Strategies
- Sequential FLP then Cre recombination
- Intersectional approaches for precise targeting
- Conditional rescue in Cre defined populations
Gene Expression Control
- FRT Stop FRT cassettes block downstream expression
- Tissue specific activation via FLP driver lines
- FLP dependent reporter activation for lineage tracing
FLP FRT vs Cre Lox
FLP Variants and Driver Lines
FLPe (Enhanced)
Four point mutations improve stability at 37°C, approximately 4 fold higher efficiency
FLPo (Optimized)
Codon optimized for mammalian expression, highest recombination efficiency
FlpERT2 (Inducible)
Tamoxifen inducible FLP for temporal control of recombination
What Researchers Say
“The Hephaestin flox model ingenious has made for us has been great. It has helped generate eight research publications.”
— Joshua Dunaief, PhD, MD
University of Pennsylvania