Ingenious offers a convenient and valuable PCR genotyping service that you can take advantage of when the production of your custom mouse model is complete. This service can include any PCR assay we developed when screening the targeted mouse ES cells or genotyping the mice during our production of your knockout or knockin mouse model.
Our service provides unambiguous detection of your targeted allele, whether it is the primary allele or the recombined allele after Cre or Flp activity. We can help you identify which mice you need to keep from the new generations of mice that you generate from breeding your custom mouse model.
Examples of services that require one PCR assay each:
Screening for Neo selection cassette deletion
Screening for distal loxP (heterozygous or homozygous conditional knockout)
Screening for Sox2-Cre transgene
Screening for FLP deleter transgene
Screening for heterozygous ROSA26 knockin
Screening for heterozygous conditional inversion knockin
Examples of services that require two PCR assays each:
Screening for homozygous ROSA26 knockin
Screening for homozygous conditional inversion knockin
Example of services that require one PCR assay & DNA sequencing each:
Screening for constitutive point mutation knockin
The service fee per tail includes DNA extraction, PCR, gel analysis, and data report. We can also provide a more customized genotyping service involving PCR assay development and primer design. Note that a more customized genotyping service may require a minimum number of samples to be provided (e.g., five or more samples).
The genotyping report will include:
Schematic showing the location of the primers
Sequences of the primers
Photos of PCR gel results
Table of PCR results for the tail samples submitted
If you would like tail DNA samples shipped back to you, please indicate this in the sample submission form. We will store the tail DNA samples for up to three weeks, unless you specifically request a longer storage period.
The genotyping report is provided upon receipt of payment for the service. Click here to view an example genotyping report.
After tail DNA extraction, the DNA quality and quantity is measured. If DNA degradation is found in the sample, such as due to issues related to the tail-tipping process or storage or transport of the tail snips prior to receipt by us, PCR cannot be performed with the sample.
We will automatically repeat a failed PCR assay at no additional cost to you.
For best processing results, your tail snips should be uniform in size, about 2 to 3 mm per tail sample.
Your tail tips should be placed individually in tubes and capped tightly, and either frozen in dry ice or immersed in 70-95% ethanol as soon as possible after the tail tips have been collected. If the tail tips are frozen, ensure that they are shipped in dry ice. If the tail tips are in ethanol, they can be stored at room temperature and shipped using FedEx, UPS, or standard mail.
Use an alcohol-resistant marker to clearly label the side or top of each tail sample tube with a Tube ID that corresponds to the Tube ID that you will enter in the sample submission form.
Please download this genotyping submission form, enter your sample and order information, and then submit it to us for your genotyping order request. If possible, please send us your shipment tracking number when you ship your tail samples.
If you need assistance for selecting the appropriate genotyping service for our mouse tail samples, please contact Mikaela Vallas at firstname.lastname@example.org prior to submitting your genotyping order request.
We accept Purchase Orders, credit card, and Apple Pay for this service.