Conditional Knockout Mouse Models

Conditional Knockouts

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Conditional Knockout Mouse

A conditional knockout mouse model lets you control where and when your gene of interest is knocked out.  A conditional knockout model initially expresses the wild-type sequence. The target gene is only knocked out in cells where a specific recombinase is active. You control where knockout occurs by crossing two lines together: your conditional knockout line and a recombinase-expressing line.  Cre recombinase is the enzyme most commonly used for conditional genetic rearrangements. It recognizes specific sequences known as lox sequences, e.g. loxP. Other recombinase/recognition sequence pairs are also used such as FLP/FRT and Dre/rox.

When you receive your new conditional line from ingenious,  it can be used in conjunction with hundreds of existing Cre lines for precise regulation. A conditional approach limits the knockout to your tissue of interest, avoiding potential embryonic lethality from constitutive knockout. Using Cre lines with drug-inducible activity expands your options to include temporal control of your knockout. There are many Cre’s to choose from such as tissue specific, inducible, and ingenious’ own Split-Cre for generating lines that are not currently available. The placement of loxP sites can be tricky when considering promoter and regulatory regions, ingenious experts can help alleviate this concern. Contact us today and our experienced scientists will analyze your gene of interest and develop a targeting strategy that is perfect for your research. Learn more about a conditional knockout model.


New technologies can enhance the utility of your conditional knockout (CKO) line.

ingenious’ proprietary No-Doubt Conditional Knockout™ system can activate expression of a reporter gene

in cells where your gene is knocked out independent of native gene promoter activity. 

Enhance your conditional model by adding a reporter to label affected cells, &  for visualizing where your gene has been knocked out on the cellular level.



Our 4 conditional knockout model variations:


NO-DOUBT CONDITIONAL Knockout™

To address the critical need of researchers to verify the loxP-mediated recombination of their conditional knockout models, and to do so in a reliable manner without increasing timelines, ingenious has developed the No-Doubt Conditional Knockout™ system.

KO FIRST- MULTIPLE MODELS FROM ONE

Generating one mouse line with the potential for producing multiple derivative mouse models is a great way to save time and money. A knockout-first mouse model – with or without a reporter – can be used in gene rescue experiments and can also include conditional knockout potential.



Cre-Lox Facts

6 Facts You May Not Know About Cre-Lox By Richard Row, PhD The basics of conditional knockout mice are familiar to any researcher who uses mouse models, even if this model type isn’t yet a part of your research. Using the Cre-lox system in an experiment seems simple: two lines are crossed, one with tissue-specific

Gaining Inducible Control

Inducible control of your tissue-specific animal model by Kristen Couglin The Cre-lox system is commonly used to provide tissue-specific gene knockout.  In order for this to happen in vivo, two mouse lines are mated together – The tissue-specific Cre line and a second mouse line containing your gene of interest flanked by loxP sites (floxed

Mouse Model of Lymphoma

Taking control with a conditional point mutation model When designing a point mutation mouse model you should ask yourself, “Is a mouse with this mutation present in every cell the best model for my research?” It may make more sense to only express mutant sequence in specific cells, for example when making a cancer model.  There

“iTL has generated three knockout and conditional KO mice for my lab. One of them is now published and we would like to thank iTL for the very nice service.”

-Kai Ge, PhD