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Conditional Knockout Mouse
A conditional knockout mouse model lets you control where and when your gene of interest is knocked out. A conditional knockout model initially expresses the wild-type sequence. The target gene is only knocked out in cells where a specific recombinase is active. You control where knockout occurs by crossing two lines together: your conditional knockout line and a recombinase-expressing line. Cre recombinase is the enzyme most commonly used for conditional genetic rearrangements. It recognizes specific sequences known as lox sequences, e.g. loxP. Other recombinase/recognition sequence pairs are also used such as FLP/FRT and Dre/rox.
When you receive your new conditional line from ingenious, it can be used in conjunction with hundreds of existing Cre lines for precise regulation. A conditional approach limits the knockout to your tissue of interest, avoiding potential embryonic lethality from constitutive knockout. Using Cre lines with drug-inducible activity expands your options to include temporal control of your knockout. There are many Cre’s to choose from such as tissue specific, inducible, and ingenious’ own Split-Cre for generating lines that are not currently available. The placement of loxP sites can be tricky when considering promoter and regulatory regions, ingenious experts can help alleviate this concern. Contact us today and our experienced scientists will analyze your gene of interest and develop a targeting strategy that is perfect for your research.
New technologies can enhance the utility of your conditional knockout (CKO) line.
ingenious’ proprietary No-Doubt Conditional Knockout™ system can activate expression of a reporter gene
in cells where your gene is knocked out independent of native gene promoter activity.
Enhance your conditional model by adding a reporter to label affected cells, & for visualizing where your gene has been knocked out on the cellular level.
Our 4 conditional knockout model variations:
To address the critical need of researchers to verify the loxP-mediated recombination of their conditional knockout models, and to do so in a reliable manner without increasing timelines, ingenious has developed the No-Doubt Conditional Knockout™ system.
KO FIRST- MULTIPLE MODELS FROM ONE
Generating one mouse line with the potential for producing multiple derivative mouse models is a great way to save time and money. A knockout-first mouse model – with or without a reporter – can be used in gene rescue experiments and can also include conditional knockout potential.
“iTL has generated three knockout and conditional KO mice for my lab. One of them is now published and we would like to thank iTL for the very nice service.”
-Kai Ge, PhD