Conventional / global / constitutive KO
2,800+
Models Generated
800+
Publications
26+
Years Experience
100%
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Mouse model generation for high priority research genes
ingenious targeting laboratory designs knockout, conditional knockout, knockin, humanized, and transgenic models for 700+ priority genes across signaling, immune, cancer, neuroscience, metabolism, and morphogen pathways. Quote in 24 hours. 100% germline transmission guarantee.
Model modification types
PI taxonomy for model generation
Associate your project with the full PI search taxonomy, from knockout through backgrounds.
Knockout
Conditional KO (floxed / loxP)
Inducible conditional KO (CreER / tet)
Tissue specific KO
Specify tissue or Cre driver on quote
Knockout first (tm1a / IKMC)
Convertible floxed allele pathway
Double / compound KO
Multi allele / compound knockout project
Large scale / BAC deletion
BAC scale deletion or targeting
Knockin
Point mutation KI
cDNA KI
Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)
Tag KI (FLAG, HA, Myc, V5)
Conditional KI (e.g. Rosa26 LSL)
LSL or conditional expression knockin
Gene replacement KI
Humanized
Full gene replacement
Partial / domain humanized
Domain or partial humanization scope
Immune checkpoint humanized
Checkpoint IO humanization when gene is a checkpoint target
Single humanized
Double / multi humanized
Multi humanized / combination IO project
Transgenic
Random integration transgenic
Safe harbor (ROSA26, HPRT, H11, Col1a1)
Overexpression transgenic
BAC transgenic
BAC transgenic or large fragment insert
Cre / recombinase
Constitutive Cre driver
Inducible Cre (CreER / tet)
Tamoxifen or dox inducible Cre
Dual recombinase (Cre+Dre / Cre+Flp)
Dual recombinase breeding scheme
Flp driver
Flp or FRT derivative allele pairing
Reporter
Reporter knockin (gene specific)
Standalone reporter catalog (lineage tracing, constitutive, dual reporter)
Catalog reporter lines not tied to a single gene allele
Species / background
Rat KO / KI / transgenic
Specify rat on quote
Rabbit models
Specify rabbit on quote
C57BL/6 (B6J / B6N)
Background substrain on quote
BALB/c
129, NOD, NSG, FVB, CD1, DBA/2
Background strain on quote
Browse by research cohort
Open a gene hub for catalog paths, or request a quote for that mouse symbol.
158 genes in Core signaling and cell cycle
Search priority genes
Filter by human symbol, mouse symbol, or alias
Knockout Mouse Models
Knockout mice carry targeted deletions that eliminate gene function, enabling loss of function studies across every therapeutic area. ingenious targeting laboratory offers multiple knockout strategies for your experimental requirements.
Conventional Knockout
Conventional knockout models carry constitutive null alleles that eliminate gene function in all tissues from the earliest developmental stages. These models provide straightforward interpretation of genes that are not required for embryonic viability.
Learn MoreConditional Knockout
Conditional knockout models use the Cre lox system to enable tissue specific or temporally controlled gene deletion. The floxed allele design preserves normal gene function until Cre recombinase excises a critical region, providing experimental control over when and where knockout occurs.
Learn MoreTissue Specific Knockout
By crossing floxed alleles with tissue specific Cre driver lines, researchers can study gene function in specific cell populations while maintaining normal function elsewhere. This approach is essential when conventional knockout causes embryonic lethality or systemic effects that obscure tissue specific phenotypes.
Learn MoreInducible Knockout
Inducible systems such as tamoxifen activated CreERT2 allow temporal control over gene deletion. This enables study of gene function in adult animals and avoids developmental compensation that can mask phenotypes in constitutive models.
Learn MoreKnockout First / Convertible Allele
Knockout first designs (tm1a / IKMC style) deliver a convertible pathway from a reporter tagged null allele to a conditional floxed allele, supporting flexible study designs from a single targeting event.
Learn MoreCompound / Double Knockout
Multi allele and compound knockout projects combine two or more targeted loci for pathway redundancy, synthetic lethality, and complex disease modeling on defined backgrounds.
Learn MoreLarge Scale BAC Deletion
BAC scale deletion and large fragment targeting remove extended genomic regions when conventional exon deletion is not sufficient for your research question.
Learn MoreKnockin Mouse Models
Knockin mice carry precise sequence insertions at defined genomic locations. Unlike random transgenesis, knockin targeting ensures predictable expression levels and eliminates position effects.
Point Mutation Knockin
Point mutation models introduce specific nucleotide changes to study disease associated variants, alter protein function, or modify regulatory elements.
Learn MoreReporter Knockin
Reporter knockin models express fluorescent proteins, enzymatic markers, or other reporters under control of endogenous regulatory elements. This enables visualization of gene expression patterns, lineage tracing, and cell isolation based on marker expression.
Learn MoreTag Knockin
Tag knockin models add epitope tags such as FLAG, HA, or V5 to endogenous proteins. These tags enable protein detection, purification, and interaction studies without requiring gene specific antibodies.
Learn MorecDNA Knockin
cDNA knockin replaces a gene with a coding sequence, often to express modified proteins, isoform variants, or humanized sequences. The targeted approach ensures expression under endogenous regulatory control.
Learn MoreConditional Knockin
Conditional knockin designs such as Rosa26 LSL enable inducible or tissue restricted expression of inserted sequences while preserving baseline physiology until recombination.
Learn MoreGene Replacement Knockin
Gene replacement knockin substitutes mouse coding sequence with a human or engineered ortholog at the endogenous locus for preclinical target fidelity.
Learn MoreHumanized Mouse Models
Humanized mice carry human gene sequences in place of mouse orthologs, enabling preclinical testing of human specific therapeutics and study of human disease mechanisms.
Gene Replacement Humanization
Complete gene replacement substitutes the entire mouse gene with its human ortholog, including regulatory elements that control expression. This approach preserves physiological expression patterns while providing human target sequences.
Learn MoreImmune Checkpoint Humanization
Humanized immune checkpoint models express human PD1, PDL1, CTLA4, LAG3, TIM3, or other checkpoint proteins. These models enable testing of checkpoint inhibitor antibodies in immunocompetent mice with functional immune systems.
Learn MoreReceptor and Target Humanization
Therapeutic antibodies often show species specificity that prevents testing in standard mouse models. Humanizing the target receptor or protein enables preclinical efficacy studies in physiologically relevant contexts.
Learn MoreBAC Transgenesis
Bacterial artificial chromosome (BAC) targeting enables integration of large genomic segments including complete genes with native regulatory elements. This approach is valuable when physiological expression patterns are essential.
Learn MorePartial / Domain Humanization
Partial or domain humanization replaces selected exons or functional domains when full gene replacement is unnecessary, reducing project scope while preserving key human epitopes or binding sites.
Learn MoreDouble / Multi Humanized
Double and multi humanized projects combine two or more humanized loci for combination IO, receptor pairs, or multi target therapeutic programs on a single defined background.
Learn MoreTransgenic and Cre Driver Models
Random and safe harbor transgenesis, overexpression alleles, BAC inserts, and Cre driver lines complete the PI search taxonomy for model generation beyond targeted knockout and knockin.
Transgenic Mouse Service
Random integration and overexpression transgenic models for rapid gain of function studies, with project design guidance on copy number and founder screening.
Learn MoreSafe Harbor Rosa26 / H11
Targeted insertion at Rosa26, H11, and related safe harbor loci for predictable expression without disrupting essential genes.
Learn MoreCre Recombinase Drivers
Constitutive and inducible Cre driver lines for tissue specific or temporal control of floxed alleles across conditional knockout and knockin programs.
Learn MoreCustom Transgenic Quote
Request a quote for BAC transgenic, overexpression, or dual recombinase breeding schemes tailored to your allele plan.
Learn MoreThe ingenious targeting laboratory Approach
Scientific Consultation
Every mouse model generation project begins with scientific consultation. Our team reviews your research goals, evaluates targeting strategy options, and recommends the approach most likely to deliver the experimental capabilities you need.
Comprehensive Documentation
Every project includes detailed documentation of targeting design, germline transmission records, and genotyping protocols. This documentation supports ongoing research and future breeding programs.
What Researchers Say
“We are actively engaged in the production of a number of conditional mouse models with iTL. This collaboration has been simple on my end, just providing the gene accession numbers of each gene, and iTL recommending the strategies for each gene. The full range of mouse knockout services matches my needs well. I find iTL's service uniquely useful for my situation of needing different models for my research in a quick and efficient manner.”
— Mehboob Hussain, MD
University of Michigan Health
Selected Publications
Mouse model generation from ingenious targeting laboratory have contributed to over 800 peer reviewed publications.
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