Conditional Knockin Mice

Conditional knockin models include LoxP-flanked stop cassettes or other conditional elements that prevent expression until Cre-mediated recombination occurs. This enables tissue-specific or temporal control of knockin expression. Regular knockin models express the inserted sequence constitutively in all tissues.

Use conditional knockin when the inserted sequence (e.g., point mutation, reporter, humanized gene) would cause lethality or severe phenotypes if expressed globally, or when you need tissue-specific or temporal control. Conditional knockin enables study of gene function in specific cell types or at specific timepoints.

Expression is controlled through Cre recombinase. Tissue-specific Cre drivers enable spatial control. Tamoxifen-inducible Cre (CreER) enables temporal control. After Cre-mediated recombination, the conditional element is removed and expression begins. Expression is permanent once recombination occurs.

Conditional knockin can insert point mutations, reporters (GFP, luciferase), epitope tags, humanized gene sequences, or cDNA sequences. The conditional cassette (LoxP-flanked stop) prevents expression until Cre recombination removes it, enabling controlled expression of the inserted sequence.

Custom model generation timelines vary based on project complexity and specific requirements. Contact us for a detailed timeline estimate tailored to your conditional knockin project.