Pathway Analysis Mouse Models

Systematic pathway analysis typically targets 5 to 20 pathway components depending on pathway complexity and research question scope. Comprehensive analysis requires examining single component knockouts, compound knockouts of redundant genes, and therapeutic target knockouts. Larger numbers require more extensive breeding and phenotyping.

Compound knockout of suspected redundant genes provides direct functional assessment. Expression analysis revealing compensatory upregulation of related genes in single knockouts indicates redundancy. Double knockout rescue through transgenic overexpression confirms redundancy mechanisms.

Yes, tissue specific conditional knockouts combined with inducible Cre enable both temporal and spatial pathway control. These approaches enable investigation of stage specific and tissue specific pathway functions.

Reporter knockins reveal spatial and temporal expression patterns of pathway components enabling correlation with functional requirements. Multi color reporters permit simultaneous visualization of multiple pathway genes.

Co-expression analysis examining which genes are expressed together reveals likely pathway interactions. Network analysis constructing interaction networks from functional and physical evidence identifies pathway structure. Machine learning prediction of genetic interactions guides experimental pathway analysis.