Skip to main content
Health Reestablishment

Rederivation Services

ingenious targeting laboratory provides mouse rederivation services to reestablish specific pathogen free (SPF) health status for valuable mouse lines. Since 1998, we have successfully rederived hundreds of mouse lines, enabling transfer to SPF facilities and restoration of colony health.

Rederivation eliminates pathogens through embryo transfer or cesarean section, producing offspring free of maternal pathogens. This process is essential when mouse lines have been exposed to pathogens or when transferring lines to SPF facilities requiring specific health status.

Request a Quote→Talk to a Scientist→
2,500+
Projects Completed
800+
Publications
26+
Years Experience
100%
Success Rate

Rederivation Methods

Embryo Transfer

Embryo transfer is the preferred rederivation method:

  • Collection:Harvest embryos from donor females
  • Washing:Multiple washes remove surface pathogens
  • Transfer:Transfer to SPF recipient females
  • Advantages:Most reliable, preserves genetic integrity
  • Timeline:3 to 4 weeks to weaning

Cesarean Section

Cesarean section provides alternative rederivation:

  • Procedure:Surgical delivery from SPF recipient
  • Cross Fostering:Transfer to SPF foster mothers
  • Applications:When embryo transfer not feasible
  • Timeline:Similar to embryo transfer

When Rederivation is Needed

Health Status Requirements

Rederivation required when:

  • Transferring to SPF facilities
  • Pathogen exposure occurred
  • Health status unknown
  • Facility health requirements change

Pathogen Elimination

Rederivation eliminates:

  • Viral Pathogens:MHV, Sendai, MPV
  • Bacterial Pathogens:Mycoplasma, Helicobacter
  • Parasites:Pinworms, mites
  • Other Pathogens:As specified by facility

Service Process

1

Pre Rederivation

Initial assessment includes:

  • Health status evaluation
  • Genetic verification
  • Method selection
  • Timeline planning
2

Rederivation Procedure

Procedure includes:

  • Donor preparation
  • Embryo collection or cesarean
  • Transfer to SPF recipients
  • Monitoring and care
3

Post Rederivation

Following rederivation:

  • Health monitoring
  • Genetic verification
  • Colony establishment
  • Health documentation
✦ New for 2026

Breeding Scheme Architect

Plan complex multi-allele breeding strategies, calculate expected genotype ratios, and estimate time to experimental cohorts—all before starting your project.

✓
Visualize multi-generation breeding paths
✓
Calculate Mendelian ratios automatically
✓
Estimate timeline to study-ready cohorts
Try It FreeGet Expert Help

Free Research Tool

No account required

Allele 1Gene-flox (conditional)
Allele 2Cre-driver (tissue-specific)
TargetHomozygous knockout

→ 3 generations to target genotype

What Researchers Say

“We found iTL's service to be exceptionally efficient and very well managed. We generated several mouse lines with iTL, and every step for each project was performed quickly and precisely, without any delays or problems.”

— Santos Franco, PhD

University of Colorado Anschutz Medical Campus

Request Rederivation Services

Contact us to discuss rederivation requirements for your mouse lines. Our team can help you determine the optimal approach for reestablishing SPF health status.

Request a Quote→Free Consultation→

Frequently Asked Questions

Rederivation is the process of generating pathogen-free mice from existing lines through embryo transfer or IVF into clean recipient females. It's needed when mouse lines become contaminated with pathogens, need to be moved to SPF facilities, or require health status upgrade for specific research protocols.

Rederivation methods include embryo transfer (collecting embryos from donor females and transferring to clean recipients), IVF (in vitro fertilization with clean sperm and eggs, then embryo transfer), and cesarean section (surgical delivery of pups to clean foster mothers). Method selection depends on line characteristics and pathogen status.

Post-rederivation health testing includes screening for standard mouse pathogens (viruses, bacteria, parasites), confirmation of pathogen-free status, and documentation of health certificates. Testing ensures rederived lines meet SPF facility requirements and are suitable for research use.

Yes. Rederivation preserves the genetic integrity of mouse lines including complex genotypes, multiple alleles, and transgenic combinations. Embryo transfer or IVF methods maintain all genetic modifications while establishing pathogen-free status. Genotyping confirms genotype preservation after rederivation.

Lab Signals

Rederivation Service Insights

Expert research insights delivered biweekly. Written by PhD scientists, designed for researchers who need cutting-edge knowledge to advance their projects.

Subscribe Free

Related Services

Resources