Cdh5 Transgenic mouse models
Vascular endothelial cadherin is encoded by Cdh5, ortholog of human CDH5, a classical cadherin restricted to endothelium whose five extracellular repeats mediate calcium-dependent homophilic adhesion at adherens junctions while the cytoplasmic tail binds p120 catenin to block internalization and beta-catenin to link alpha-catenin and the actin cytoskeleton. VEGF-driven Src and Vav2 phosphorylation of specific tail tyrosines triggers junction disassembly and permeability, making this locus the molecular control point for vascular leak. Germline deletion is lethal near midgestation from failed vascular remodeling rather than failed vasculogenesis, so conditional alleles are mandatory, and phosphosite knockins that lock or mimic tyrosine modification separate adhesion from signaling far better than a null. Because Cdh5-CreERT2 is the field standard pan-endothelial driver, confirm that your floxed allele and driver are not competing at the same locus. Assess Evans blue extravasation, junction morphology by immunofluorescence, and retinal angiogenesis.
Transgenic approaches add copies of Cdh5 under a chosen promoter or as a genomic BAC. They help when you need graded overexpression or locus independent deployment.
What Cdh5 Transgenic mouse models are available?
ingenious targeting laboratory offers 1 distinct Cdh5 transgenic catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Cdh5-Cre-Tg(NMX) | Transgenic | Cre/Dre Toolbox of Mice, random transgenic mice | live | TG 192013 | Inquire |
Why this approach
We help teams pick alleles that match the experimental question, the organ system, and future breeding plans. For your target tissue centric work, Cre specificity, flox placement, and baseline controls matter as much as the gene edit itself.
Pricing and quotes
The Cdh5 Transgenic lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Cdh5 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Cdh5 Transgenic mouse models are available?
When Cdh5 Transgenic lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Cdh5 knockout embryonic lethal in mice?
It depends on background and allele design. Some Cdh5 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Cdh5 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Cdh5?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.