Alfp-Cre for liver conditional models
Tissue restricted knockout of Target keeps wild type function everywhere else. Breeding is often more robust, and the setup mirrors patient biology where mutations arise in a subset of cells. For liver work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Driver pairing notes
Alfp-Cre biases recombination toward liver lineages. Inducible design: no, constitutive activity.
Conditional knockout focuses the experiment on liver while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
A conventional knockout answers whether the gene is required broadly. When liver is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Example conditional alleles to pair with Alfp-Cre
Frequently asked questions
What animals express Alfp-Cre?
Alfp-Cre is used for liver biased recombination in community standard protocols. We recommend reporter validation on your background before large experiments.
Is Alfp-Cre inducible?
Some lines in the CreERT2 family need tamoxifen for nuclear access. Tell us your timing goals and we help pick tamoxifen versus constitutive strategies.
Which floxed genes pair with Alfp-Cre?
Top pairs depend on your disease model. We link common conditional alleles in our catalog and can suggest three to five references genes that match liver biology.