Vav1-Cre for hematopoietic conditional models
Conditional deletion of Target limits genetic change to the lineage you choose. That precision matters for oncology, immunology, and metabolic work where systemic loss would confound interpretation. After you confirm your Cre specificity, crossing to Target floxed stock yields interpretable cohorts. For hematopoietic work, plan Cre specificity, reporter crosses, and baseline phenotyping before you scale.
Driver pairing notes
Vav1-Cre biases recombination toward hematopoietic lineages. Inducible design: no, constitutive activity.
Conditional knockout keeps hematopoietic as the experimental theater while the rest of the animal retains a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
A conventional knockout answers whether the gene is required broadly. When hematopoietic is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Example conditional alleles to pair with Vav1-Cre
Frequently asked questions
What animals express Vav1-Cre?
Vav1-Cre is used for hematopoietic biased recombination in community standard protocols. We recommend reporter validation on your background before large experiments.
Is Vav1-Cre inducible?
Some lines in the CreERT2 family need tamoxifen for nuclear access. Tell us your timing goals and we help pick tamoxifen versus constitutive strategies.
Which floxed genes pair with Vav1-Cre?
Top pairs depend on your disease model. We link common conditional alleles in our catalog and can suggest three to five references genes that match hematopoietic biology.