Double Immune Checkpoint Mice

Combinations include PD1 + CTLA4, PD1 + LAG3, PD1 + TIM3, CTLA4 + LAG3, and other paired checkpoint humanizations. Selection depends on your research question and therapeutic targets. Ingenious targeting laboratory can combine any two checkpoint humanizations for combination immunotherapy studies.

Double checkpoint models are generated through sequential targeting and breeding. First the initial immune checkpoint is humanized. Then, the second immune checkpoint is humanized on the same background, followed by breeding to combine both humanizations. Careful genotyping ensures both alleles are maintained throughout breeding.

Double immune checkpoint models enable testing of combination checkpoint blockade therapies in a single model system. This is important because combination therapies (e.g., PD1 + CTLA4) show enhanced efficacy in clinical trials. Testing combinations in double humanized models provides more physiologically relevant preclinical data.

Combining two checkpoints requires sequential targeting (if starting from scratch) plus breeding time to generate double-humanized animals. If single-humanized models already exist, combining them requires only breeding time. We also have a catalog of already-created double immune checkpoint models that are maintained as live colonies for quick distribution. Contact us to determine the timeline for your specific model of interest.

Yes. Ingenious targeting laboratory can combine three or more checkpoint humanizations for complex immunotherapy combination studies. Multiple humanizations require careful breeding and genotyping to maintain all alleles. Triple-checkpoint models enable testing of triple combination therapies or more sophisticated experimental designs.