Mouse Model Types
Knockout Mouse Models
Knockout mice enable loss of function studies by eliminating specific gene activity. ingenious targeting laboratory offers both conventional knockouts for complete gene inactivation and conditional knockouts that allow spatial and temporal control of gene deletion.
Conventional Knockout
Permanently disrupt target gene function in all tissues throughout development, ideal for understanding essential gene function and creating disease models where complete loss of function is required.
Conditional Knockout
Use the Cre lox system to enable gene deletion in specific tissues or at defined timepoints, essential for studying genes with embryonic lethal phenotypes or investigating tissue specific gene function.
Knockin Mouse Models
Knockin mice introduce precise genetic modifications at defined loci, enabling reporter expression, point mutations, tag insertions, and gene humanization strategies.
cDNA Knockin
Replace endogenous genes with alternative coding sequences, enabling expression of modified or humanized genes under endogenous regulatory control.
Point Mutation
Introduce specific nucleotide changes to model human disease variants or study protein function with surgical precision.
Reporter Knockin
Insert fluorescent proteins, enzymatic reporters, or other detection systems to visualize gene expression patterns and cell populations in vivo.
Humanized Mouse Models
Humanized mice replace mouse genes or gene segments with their human counterparts, creating translational platforms for therapeutic development and human disease modeling.
Gene Replacement
Substitute entire mouse genes with human orthologs, enabling testing of human specific therapeutics and studying human gene function in vivo.
Immune Checkpoint
Create mice expressing human PD1, PDL1, CTLA4, LAG3, TIM3, and other checkpoint molecules for immunotherapy development.
Receptor Humanization
Enable preclinical testing of biologics targeting human proteins that lack mouse cross reactivity.
Transgenic Mouse Models
Transgenic mice carry additional genetic sequences integrated into the genome, enabling overexpression studies, reporter lines, and Cre driver strains.
Targeted Transgenic
Use safe harbor loci including Rosa26, HPRT, and Polr2a to achieve predictable, single copy integration with stable expression across generations.
Advanced Technologies
Conditional Gene Targeting
ingenious targeting laboratory specializes in conditional allele design using the Cre lox recombination system. Our derivative allele approach creates flexible alleles that can generate multiple model types from a single targeted ES cell clone.
The tm1a knockout first allele can be converted to:
This flexibility maximizes research value while minimizing project timelines and costs.
Learn about Cre Lox SystemStrain Background Options
ingenious targeting laboratory generates custom models on the genetic background most appropriate for your research goals:
C57BL/6
The most widely used inbred strain, with extensive phenotypic data and compatibility with most disease models.
BALB/c
Offers advantages for immunology research and certain tumor models.
129 Strain
ES cells provide exceptional targeting efficiency for complex allele designs.
Mixed Backgrounds
Can be backcrossed to your preferred strain following model generation.
What Researchers Say
“The Hephaestin flox model ingenious has made for us has been great. It has helped generate eight research publications.”
— Joshua Dunaief, PhD, MD
University of Pennsylvania
Additional Services
Genotyping Services
ingenious targeting laboratory provides comprehensive genotyping services to support your mouse model research.
- PCR based genotyping for targeted alleles
- Quantitative PCR for copy number determination
- Sequencing based genotyping for point mutations and small insertions
Targeting Vector Design
Our scientific team designs and constructs targeting vectors tailored to your specific research requirements.
- Plasmid based vectors for standard targeting projects
- BAC based vectors for large scale targeting (up to 200kb)
- Custom cassette configurations for complex allele designs
- Validated vector backbones with proven targeting efficiency
Microinjection Services
ingenious targeting laboratory's microinjection services deliver targeted ES cells into blastocysts for chimera generation.
- Blastocyst injection with characterized ES cell clones
- Morula injection for accelerated timelines
- High efficiency injection protocols
- Quality controlled injection procedures
Start Your Project
Our scientific consultants can help you design the optimal mouse model strategy for your research goals. From initial concept through study ready animals, ingenious targeting laboratory provides comprehensive support at every project phase.
Frequently Asked Questions
Mouse Model Service Insights
Expert research insights delivered biweekly. Written by PhD scientists, designed for researchers who need cutting-edge knowledge to advance their projects.