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Metabolic Research

Obesity Mouse Models

Since 1998, ingenious targeting laboratory has supported obesity research with custom mouse models enabling mechanistic studies of energy homeostasis, adipose tissue biology, and metabolic dysfunction underlying the global obesity epidemic.

Obesity mouse models provide essential platforms for investigating the molecular pathways regulating appetite, energy expenditure, and fat storage.

2,500+
Projects Completed
800+
Publications
26+
Years Experience
100%
Success Rate

Genetic Obesity Models

Leptin (ob/ob)

Spontaneous mutation in leptin. Severe obesity due to hyperphagia and reduced energy expenditure. Established leptin as key regulator of energy balance.

Leptin Receptor (db/db)

Mutations in leptin receptor producing leptin resistance. Also used for diabetes research due to pronounced hyperglycemia.

MC4R Knockout

Melanocortin 4 receptor knockout produces severe obesity. MC4R mutations are most common monogenic cause of human obesity.

POMC Knockout

Proopiomelanocortin deficiency eliminates alpha MSH, producing obesity with pigmentation defects.

Diet Induced Obesity Models

C57BL/6 on High Fat Diet

Most common obesity model. Susceptible to diet induced obesity, insulin resistance, and fatty liver on 45 to 60% fat diet.

Genetic + DIO

Knockout or knockin alleles combined with high fat diet reveal gene diet interactions and obesity susceptibility modifiers.

Strain Comparisons

Different inbred strains show varying susceptibility. A/J mice relatively resistant compared to C57BL/6.

Tissue Specific Targets

TissueCre DriversApplications
HypothalamusNestin Cre, POMC Cre, AgRP Cre, Sim1 CreCentral appetite and energy regulation
AdiposeAdiponectin Cre, Fabp4 Cre, UCP1 CreLipid storage, thermogenesis
LiverAlbumin CreGlucose and lipid metabolism
MuscleMCK Cre, MyoD CreGlucose uptake, fatty acid oxidation

Phenotyping Endpoints

Body Composition

  • Body weight curves
  • DEXA/MRI fat and lean mass
  • Adipose depot weights

Metabolic

  • Food intake monitoring
  • Indirect calorimetry (O2, CO2)
  • Core temperature
  • Physical activity

Glucose/Lipid

  • IPGTT/OGTT
  • Insulin tolerance
  • Plasma lipids
  • Hepatic triglycerides

What Researchers Say

iTL produced a new conditional mouse model for us and the quality of service was exceptional. The team is extremely knowledgeable and the work was completed at the highest possible standards. My project manager was excellent and always happy to answer technical questions and keep me up to date with progress and potential problems. I would recommend iTL highly and will use them again in the future if I need to generate a new mouse line.

Albert Basson, PhD

King's College London

Start Your Obesity Model Project

Our scientific consultants can help design custom obesity models tailored to your research questions, whether studying central regulation, peripheral metabolism, or therapeutic interventions.

Frequently asked questions

Common targets include adipose tissue (Adiponectin Cre), hypothalamus (Nestin Cre, POMC Cre, AgRP Cre for appetite control), liver (Albumin Cre for glucose and lipid metabolism), and muscle (MCK Cre for glucose uptake). Tissue selection depends on your research question.

High fat diets typically contain 45 to 60% fat calories. Higher fat content produces more rapid weight gain. Defined diets are preferable for reproducibility. Duration (8 to 12 weeks for mild, 16+ weeks for severe obesity) and age at initiation affect phenotype development.

Standard assessments include body weight, body composition (DEXA or MRI), food intake, energy expenditure (indirect calorimetry), glucose tolerance testing, insulin tolerance testing, plasma lipid profiles, and adipose depot analysis.

Yes. Combining tissue specific gene modifications with high fat diet challenge enables study of how specific genes affect diet induced obesity and metabolic dysfunction. For example, adipose specific knockouts on high fat diet reveal adipocyte autonomous effects on systemic metabolism.

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