Core Technologies
Homologous Recombination
Homologous recombination enables precise genetic modification by exchanging genomic sequences between the targeting vector and chromosomal DNA.
- Targeted modifications occur at the intended locus
- Surrounding genomic architecture remains intact
- Single copy integration at native chromosomal position
- Predictable expression under endogenous regulatory control
Conditional Systems
Cre Lox System
The Cre lox recombination system enables conditional gene modification by placing critical exons between LoxP recognition sites. Cre recombinase expression catalyzes recombination between LoxP sites to delete the flanked sequence.
Learn moreFLP FRT System
The FLP FRT system provides an orthogonal recombination approach that can work independently or in combination with Cre lox for selection cassette removal, sequential recombination, and dual recombinase strategies.
Learn moreInducible Systems
Inducible gene expression systems provide temporal control over genetic modifications. Tamoxifen inducible CreERT2 systems enable adult onset gene deletion or activation.
Learn moreDerivative Allele System
The knockout first strategy produces a tm1a allele that serves as the foundation for multiple model types from a single targeting event.
tm1a Allele (knockout first)
Gene function disrupted by splice acceptor. LacZ reporter indicates endogenous expression pattern.
tm1b Allele (complete null)
Cre mediated deletion removes critical exon and neomycin cassette. Clean null allele with persistent reporter expression.
tm1c Allele (conditional ready)
FLP mediated deletion removes gene trap cassette, restoring gene function. Critical exon remains flanked by LoxP sites.
tm1d Allele (conditional null)
Cre mediated deletion of the tm1c allele removes the critical exon, creating tissue specific or temporally controlled null allele.
What Researchers Say
“We are very happy with this mouse line and we're not done yet. There's still plenty to do. The versatility of the F.A.S.T.™ approach is really unparalleled. There are so many possibilities to use the cassette that one paper does not showcase it all. Also, the addition of N-terminal GFP has helped quite a bit to understand gene expression patterns.”
— Frank Bosmans, PhD
Ghent University, Belgium
Start Your Project
Our scientific consultants can help you select the optimal technology approach for your research goals. From allele design through study ready animals, ingenious targeting laboratory provides comprehensive technical expertise.