Kcnj14 Knockout mouse models
Encoding Kir2.4, Kcnj14 is the mouse ortholog of human KCNJ14 and produces the most restricted member of the strong inward rectifier Kir2 subfamily, expressed predominantly in retinal neurons, cranial and spinal motor neurons, and some cardiac tissue. Like its relatives it rectifies through voltage-dependent polyamine and magnesium block and requires PIP2 for opening, but Kir2.4 is distinguished by higher sensitivity to extracellular pH within the physiological range and by lower single-channel conductance, features that make it a tunable contributor to resting potential in motor neurons rather than a bulk conductance. It readily co-assembles with Kir2.1, Kir2.2, and Kir2.3 to form heteromeric channels with intermediate properties. Because the encoded subunit is a minority component in most tissues, conditional deletion alone often yields little phenotype, and compound alleles with Kcnj2 or Kcnj12 plus dominant-negative pore knockins are the practical route; select motor neuron or retina-restricted Cre drivers to align the allele with the intended readout.
Whole body loss of Kcnj14 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Kcnj14 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Kcnj14 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kcnj14-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 200283 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Kcnj14 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kcnj14 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kcnj14 Knockout mouse models are available?
When Kcnj14 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kcnj14 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kcnj14 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kcnj14 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kcnj14?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.