Mouse Backcrossing Services
Since 1998, ingenious targeting laboratory has provided backcrossing services to transfer targeted alleles onto defined genetic backgrounds, ensuring consistent phenotypes and compatibility with established research strains. Backcrossing is essential for eliminating mixed background effects, enabling strain specific phenotyping, and generating mice compatible with existing colony backgrounds used by research programs worldwide.
Frequently asked questions
Traditional backcrossing takes 10 generations (approximately 2.5 years) to reach 99.9% background purity. Speed congenic uses SNP panel analysis to identify offspring with the highest recipient background content at each generation, reducing the process to 5-7 generations (approximately 1-1.5 years).
Congenic status (>99.9% recipient background) requires N10 using traditional backcrossing. Speed congenic approaches can achieve equivalent purity in N5-N7 generations through marker-assisted selection of optimal breeders.
Common target backgrounds include C57BL/6J, C57BL/6N, BALB/c, and other inbred strains. C57BL/6 is most frequently requested due to extensive phenotypic characterization and compatibility with many experimental paradigms.
Yes, but complexity increases with each additional allele. Each allele must be genotyped at every generation. For lines with multiple alleles, we recommend backcrossing alleles separately when possible, then intercrossing congenic lines.
Genetic background influences phenotype. Modifier loci can enhance or suppress phenotypes, causing variability between labs using different backgrounds. Congenic mice enable reproducible experiments and valid comparisons with published data on the same background. (/request-quote)
