Pycard Knockout mouse models
Pycard, the mouse ortholog of human PYCARD, encodes ASC, a bipartite adaptor whose pyrin domain nucleates prion-like filaments with activated sensors including NLRP3, NLRC4, AIM2, and pyrin, while its CARD recruits and clusters pro-caspase-1 into the dense speck where proximity-induced autoproteolysis occurs. Mature caspase-1 then processes IL-1 beta and IL-18 and cleaves gasdermin D to open plasma membrane pores, and ASC specks released from dying cells continue to mature cytokines extracellularly, making ASC a signaling node rather than a passive linker. Nulls are viable and fertile, so straight deletion cleanly reports inflammasome dependence, conditional alleles assign requirement to macrophages, neutrophils, or epithelium, and phosphosite or filament-disrupting knockins separate speck assembly from caspase recruitment. Certain NLRC4 and NLRP1 responses proceed with reduced ASC dependence, so match the allele to endpoints such as IL-1 beta release, pyroptosis kinetics, or gout and peritonitis models.
Global Pycard deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Pycard Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Pycard knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Pycard-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 231704 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Pycard Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Pycard allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Pycard Knockout mouse models are available?
When Pycard Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Pycard knockout embryonic lethal in mice?
It depends on background and allele design. Some Pycard germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Pycard animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Pycard?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.