BAC to BAC: Large Scale Gene Targeting

Break free from targeting size constraints with our BAC technologies.

For too long, mouse model designs have been dictated by size constraints imposed by the limitations of technology. At ingenious targeting laboratory, our mission to push past limits has led to the development of proprietary BAC and fosmid technologies that reduce the time associated with these projects to a fraction of their typical imposing scale.

By offering a standard 200kb target size, with no restrictions, researchers are now empowered to design models tailored to their specific research needs without limitations. Our efficient BAC targeting also streamlines the process, allowing researchers to go from project concept to confirmed F1 mice much faster than with traditional methods.

What Researchers Say

"ingenious was very competitively priced compared to other reputable transgenic companies. Most importantly, however, from the very beginning their knowledge and competence convinced me they would deliver on our project, so I trusted them and they fully met our expectations. Why use anyone else!"

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— Murray Clarke, PhD, University of Cambridge

Combining BAC targeting with our other technologies

TruView Conditional Knockout™: Certainty through GFP

Flank entire genes with ingenious' next generation conditional knockout system in a single targeting step. By combining reliable, long range BAC targeting with ingenious' split-GFP technology, verification of your BAC scale knockout has never been more clear.

TruHumanization™

Replace an entire genomic mouse gene with the human ortholog, including introns, in a single targeting step. Intronic sequences contain regulatory features, microRNA genes, promoters, enhancers, and splice sites for transcript isoforms. Ensure that you are working with the complete picture, because a gene is more than just the protein that it encodes.

Point Mutation Models

Introduce as many point mutations as needed in a single targeting step with a reliable timeline. Alternative approaches, such as CRISPR, require multiple independent targeting events to achieve the same result. The odds of multiple targeting events occurring on the same allele are quite low and decrease with each additional mutation required.

Knockout Models

Delete entire genes and gene clusters in a single targeting step without the fear of truncated protein expression.

Knockin Models

From co-expression to fusion, to reporter tagging and more - ingenious can achieve these designs in a single targeting step. Introduce the exogenous sequences that your research requires, not what is simply available utilizing outdated technologies.

Breaking Size Constraints

For too long, mouse model designs have been dictated by size constraints imposed by the limitations of technology. At ingenious, our mission to push past limits has led to the development of proprietary BAC and fosmid technologies that reduce the time associated with these projects to a fraction of their typical imposing scale.

From the initial project concept, to identifying positive clones, to confirming F1 neo deleted mice, all within a year - 200 kb targeting is the new "standard size" at ingenious targeting laboratory.

Design your model, the center of your research, around what is needed to advance your field, not just what someone else tells you is possible.

Related Pages

• Humanized Mice
• Gene Replacement
• p53 Mouse Models