Mouse Model Strategies & Allele Types

Humanized Mouse Models

A genetically engineered mouse in which one or more human genes, immune system components, or biological pathways have been introduced to replicate aspects of human physiology, bridging the gap between basic research and clinical translation.

Overview

Traditional mouse models provide powerful insights into gene function and disease mechanisms—but their biology can differ significantly from humans. Humanized mouse models solve this challenge by integrating human genetic material or biological components directly into the mouse genome or immune system.

Types of Humanized Models

Gene replacement models replace mouse genes with human orthologs. Immune system humanization integrates human immune components. Cell and tissue engraftment models use human hematopoietic stem cells or tumor tissues in immunodeficient mice.

Frequently Asked Questions

What's the difference between a knockin and a humanized mouse model?

A knockin mouse model introduces a new sequence into a specific genomic locus. A humanized mouse model specifically incorporates human genes, proteins, or immune components to replicate human biology.

Are humanized mice suitable for preclinical drug testing?

Yes. Humanized mice are commonly used for preclinical testing because they express human-specific targets and pathways, enabling accurate evaluation of biologics, gene therapies, and targeted drugs.