Axin1 Knockout mouse models
Axin1, the mouse ortholog of human AXIN1, encodes the concentration limiting scaffold of the beta-catenin destruction complex, using an N-terminal RGS domain to dock APC, a central segment to bind GSK3 beta and CK1 alpha, and a C-terminal DIX domain to polymerize and to engage Dishevelled DIX domains at activated LRP6 signalosomes. Its abundance is set by tankyrase mediated PARsylation and RNF146 dependent degradation, which is exactly why tankyrase inhibitors raise AXIN1 levels and suppress Wnt output. Loss of function AXIN1 mutations occur in hepatocellular carcinoma and hepatoblastoma, producing ligand-independent signaling equivalent to APC truncation. Germline null causes embryonic lethality with axis duplication in the classic Fused mutant, so hepatocyte or intestinal conditional alleles are required for tumor work. Axin2 is transcriptionally induced by Wnt and partially compensates, so double conditional stocks reveal phenotypes single deletion hides. Choose nulls for dependency and tankyrase pharmacology, and stabilization-resistant knockins to interrogate degradation control.
Global Axin1 deletion answers broad mechanism questions quickly. If timing or site matters, conditional alleles are a natural next step after the null is characterized.
What Axin1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Axin1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Axin1-KO | Knockout | KO/CKO mice | sperm cryopreservation | KO 231736 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Axin1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Axin1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Axin1 Knockout mouse models are available?
When Axin1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Axin1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Axin1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Axin1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Axin1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.