E2f1 Knockout mouse models
E2f1, mouse ortholog of human E2F1, encodes an activator E2F that heterodimerizes with DP1 or DP2 through its dimerization domain and binds TTTCCCGC consensus elements, driving the S phase transcriptional program including cyclin E, CDC6, MCM proteins and thymidine kinase. Its transactivation domain is masked by hypophosphorylated RB, and CDK4/6 and CDK2 mediated RB phosphorylation releases it at the restriction point. Uniquely among E2Fs, E2F1 is stabilized by ATM and CHK2 phosphorylation after DNA damage and induces apoptosis through ARF, TP73 and APAF1, giving it both oncogenic and tumor suppressive character, which is exactly why null mice develop tissue-specific tumors rather than being protected. Constitutive nulls are viable with thymic hyperplasia and testicular atrophy, so simple alleles work. Because E2f2 and E2f3 bind identical sites and compensate substantially, compound alleles are required for proliferation endpoints. Reserve single nulls for apoptotic threshold and damage response, and use conditional triple activator alleles for cell cycle exit studies.
E2f1 null animals are straightforward to genotype and phenotype when survival is acceptable. They remain a standard background for pharmacology, biomarker, and rescue studies.
What E2f1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct E2f1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| E2f1-KO(2) | Knockout | KO/CKO mice | sperm cryopreservation | KO 233822 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The E2f1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a E2f1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What E2f1 Knockout mouse models are available?
When E2f1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is E2f1 knockout embryonic lethal in mice?
It depends on background and allele design. Some E2f1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live E2f1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for E2f1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.