What E2f1 Knockout mouse models are available?

When E2f1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.

Is E2f1 knockout embryonic lethal in mice?

It depends on background and allele design. Some E2f1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.

Which Cre driver is best for experiments?

Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.

Do you ship live E2f1 animals?

When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.

How do I request a quote for E2f1?

Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.

●1 Model Available

E2f1 Knockout mouse model

Name: E2f1-KO(2)
Brand: ingenious targeting laboratory
SKU: KO 233822
Availability: PreOrder

Browse 1 E2f1 mouse model including knockout variants from ingenious targeting laboratory. Contact us for availability and fast turnaround.

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Why model E2f1

E2f1, mouse ortholog of human E2F1, encodes an activator E2F that heterodimerizes with DP1 or DP2 through its dimerization domain and binds TTTCCCGC consensus elements, driving the S phase transcriptional program including cyclin E, CDC6, MCM proteins and thymidine kinase. Its transactivation domain is masked by hypophosphorylated RB, and CDK4/6 and CDK2 mediated RB phosphorylation releases it at the restriction point. Uniquely among E2Fs, E2F1 is stabilized by ATM and CHK2 phosphorylation after DNA damage and induces apoptosis through ARF, TP73 and APAF1, giving it both oncogenic and tumor suppressive character, which is exactly why null mice develop tissue-specific tumors rather than being protected. Constitutive nulls are viable with thymic hyperplasia and testicular atrophy, so simple alleles work. Because E2f2 and E2f3 bind identical sites and compensate substantially, compound alleles are required for proliferation endpoints. Reserve single nulls for apoptotic threshold and damage response, and use conditional triple activator alleles for cell cycle exit studies.

What E2f1 mouse models are available?

ingenious targeting laboratory offers 1 distinct E2f1 (E2F1) catalog mouse model, featuring conditional knockout and standard knockout. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.

Model availability, allele class options, and quality control standards
Model Availability	Allele Class Options	Quality Control Standards
1 Ready Catalog Line	Conditional Knockout (cKO)	Documented Germline Transmission
Custom Model Generation	Constitutive Knockout (KO)	Strict United States QC Guidelines
Advanced Modifications	Humanized, Knockin, Transgenic	Cryo or Live Dispatch Options

Quality Control: Documented Germline Transmission
Quality Control: Strict United States QC Guidelines
Quality Control: Cryo or Live Dispatch Options

E2f1 model types

PathAvailability

Knockout
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Conditional knockout
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Knockin
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Humanized
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Transgenic / overexpression
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Prefer the order form? Order. Need an allele that is not listed? Request a quote.

E2f1 allele types

Knockout, conditional, knockin, humanized, and related paths for E2f1 (human E2F1). Catalog lines ship when inventory exists. Everything else is a generation quote.

Knockout

Conventional / global / constitutive KO

Conditional KO (floxed / loxP)

Inducible conditional KO (CreER / tet)

Tissue specific KO

Specify tissue or Cre driver on quote

Knockout first (tm1a / IKMC)

Convertible floxed allele pathway

Double / compound KO

Multi allele / compound knockout project

Large scale / BAC deletion

BAC scale deletion or targeting

Knockin

Point mutation KI

cDNA KI

Reporter KI (GFP, YFP, RFP, mCherry, tdTomato, lacZ, luciferase)

Tag KI (FLAG, HA, Myc, V5)

Conditional KI (e.g. Rosa26 LSL)

LSL or conditional expression knockin

Gene replacement KI

Humanized

Full gene replacement

Partial / domain humanized

Domain or partial humanization scope

Immune checkpoint humanized

Checkpoint IO humanization when gene is a checkpoint target

Single humanized

Double / multi humanized

Multi humanized / combination IO project

Transgenic

Random integration transgenic

Safe harbor (ROSA26, HPRT, H11, Col1a1)

Overexpression transgenic

BAC transgenic

BAC transgenic or large fragment insert

Cre / recombinase

Constitutive Cre driver

Inducible Cre (CreER / tet)

Tamoxifen or dox inducible Cre

Dual recombinase (Cre+Dre / Cre+Flp)

Dual recombinase breeding scheme

Flp driver

Flp or FRT derivative allele pairing

Reporter

Reporter knockin (gene specific)

Standalone reporter catalog (lineage tracing, constitutive, dual reporter)

Catalog reporter lines not tied to a single gene allele

Species / background

Rat KO / KI / transgenic

Specify rat on quote

Rabbit models

Specify rabbit on quote

C57BL/6 (B6J / B6N)

Background substrain on quote

BALB/c

129, NOD, NSG, FVB, CD1, DBA/2

Background strain on quote

Mouse Models

Two paths. One scientific team.

Search 14,774+ study ready strains, or commission the exact knockout, knockin, or humanized model your program requires.

Ready to ship

Search 14,774 strains. Many ship from live colonies.

Browse by gene, model type, or therapeutic area. Live colonies available for many top requested knockouts, knockins, and humanized lines.

Trp53Tumor suppressor, knockout & knockin Brca1Breast cancer model, multiple alleles Rosa26Safe harbor for reporters & cassettes

Browse Full Catalog

Model generation

Need a knockout, knockin, or humanized line?

The same U.S. scientific team designs and delivers your allele when it is not on the shelf. 100% germline transmission guarantee.

Conventional and conditional knockouts
Point mutation and humanized knockins
2,800+ models generated since 1998

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Available E2f1 catalog models

1 E2f1 line with QC documentation and technical support.

E2f1-KO(2)
Knockout
KO/CKO mice
sperm cryopreservationKO 233822
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Selected publications from ingenious targeting laboratory

Peer reviewed work involving E2f1 (E2F1) from ITL supported projects.

Kelch Repeat and BTB Domain Containing Protein 5 (Kbtbd5) Regulates Skeletal Muscle Myogenesis through the E2F1-DP1 Complex.Gong W, Gohla RM, Bowlin KM, Koyano-Nakagawa N, Garry DJ, Shi X. (2015). J Biol Chem 290: 15350-15361

View all ITL publications

Looking for a E2f1 mouse model?

We have 1 E2f1 model available — including Knockout types. Contact us today for current availability and our fastest turnaround options. All models come with full QC documentation and technical support.

Best pricing in the industry. Get a quote in 24 hours. Our team of PhD scientists is available to help you select the right model for your research.

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Models Available

Model Types

24 hrs

Quote Turnaround

Included

QC Documentation