Fus Conditional Knockout mouse models
Fus encodes the FET family RNA-binding protein FUS, ortholog of human FUS, organized as an N-terminal QGSY-rich low complexity prion-like domain, an RGG-flanked RRM, a zinc finger, and a C-terminal PY nuclear localization signal recognized by Transportin-1; it binds nascent transcripts, regulates splicing and transcription elongation, and undergoes liquid-liquid phase separation that is normally restrained by nuclear import and arginine methylation. Amyotrophic lateral sclerosis mutations cluster in the PY-NLS, notably R521C and P525L, weakening Transportin binding and driving cytoplasmic mislocalization, stress granule partitioning and progressive aggregation, a toxic gain-of-function mechanism distinct from the nuclear loss seen in FTLD-FUS. Because constitutive nulls show perinatal lethality on C57BL/6 backgrounds and only partial recovery on outbred backgrounds, NLS-mutant knockins and Δ14 frameshift alleles are the designs that reproduce motor neuron degeneration at endogenous expression. Ewsr1 and Taf15 share domain architecture and buffer nuclear functions, so score cytoplasmic mislocalization directly.
Fus conditional knockout mice carry a floxed allele so you delete function only where Cre is active. The germline allele stays intact until you cross to a tissue specific Cre. Labs reach for this design when a global knockout is lethal, when they need adult onset loss, or when regional redundancy hides a whole body phenotype.
What Fus Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Fus conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Fus-Flox | Conditional Knockout | KO/CKO mice | live | CKO 240182 | Inquire |
Designing a Fus Conditional Knockout allele
A floxed allele is needed because constitutive nulls die perinatally on C57BL/6, but the more important reason to build it is to test the loss of function hypothesis directly, and the answer so far is sobering: deleting Fus in spinal motor neurons with Chat-Cre or Mnx1-Cre is largely tolerated, which pushes causality toward cytoplasmic gain of function. Use the allele instead for nuclear loss questions relevant to frontotemporal degeneration, with Camk2a-CreERT2 or Nes-Cre, and for hematopoietic phenotypes with Vav1-Cre, since nulls show B lymphocyte defects. Flank the exons encoding the RRM through zinc finger so the product cannot retain RNA binding, and note that FUS autoregulates through retention of its own introns, so partial alleles behave unpredictably. Interpret with Mapt exon 10 splicing, intron retention signatures, neuromuscular junction morphology, and paralog levels for Ewsr1 and Taf15.
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Fus Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Fus allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Fus Conditional Knockout mouse models are available?
When Fus Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Fus knockout embryonic lethal in mice?
It depends on background and allele design. Some Fus germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Fus animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Fus?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.