Hist1h4i Conditional Knockout mouse models
Chaperone-mediated deposition determines where the histone H4 encoded by Hist1h4i, ortholog of human HIST1H4I, ends up in chromatin. The H3 to H4 dimer is escorted by ASF1A or ASF1B, which blocks premature tetramerization, then transferred to the CAF-1 heterotrimer of CHAF1A, CHAF1B, and RBBP4 for PCNA-coupled assembly behind the replication fork, or to HIRA for replication-independent gap filling with the H3.3 variant. Parental histone recycling through MCM2 and POLE3 with POLE4 preserves marks across the fork and underlies epigenetic inheritance. This locus is a standard replication-dependent cluster gene with an S phase restricted, stem-loop terminated transcript. Its invariant protein product is shared with every other H4 gene and identical to human H4, so single deletion is silent and humanization is not applicable. Use dominant tail knockins to interrogate chaperone recognition, and time Cre activity to an actively cycling population.
Conditional deletion of Hist1h4i limits the genetic change to the lineage you choose. That helps in oncology, immunology, and metabolic work where systemic loss would muddy the read. After you confirm Cre specificity, crossing to Hist1h4i floxed stock yields usable cohorts.
What Hist1h4i Conditional Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Hist1h4i conditional knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Hist1h4i-Flox | Conditional Knockout | KO/CKO mice | embryo cryopreservation | CKO 205092 | Inquire |
Why this approach
Conditional knockout focuses the experiment on your target tissue while the rest of the animal keeps a wild type allele. That pattern mirrors somatic mutation in patients and avoids systemic compensation that can erase subtle phenotypes. It is often preferred when a germline null is lethal, weak, or confounded by developmental rescue.
Pricing and quotes
The Hist1h4i Conditional Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Hist1h4i allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Hist1h4i Conditional Knockout mouse models are available?
When Hist1h4i Conditional Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Hist1h4i knockout embryonic lethal in mice?
It depends on background and allele design. Some Hist1h4i germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Hist1h4i animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Hist1h4i?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.