Kcnj1 Knockout mouse models
Kcnj1, the mouse ortholog of human KCNJ1, encodes ROMK (Kir1.1), an inwardly rectifying potassium channel built from two transmembrane helices flanking a pore loop, assembled as a tetramer, and gated by intracellular pH acting through a conserved lysine in the amino terminus together with PIP2 binding at the cytoplasmic domain. ROMK provides the apical potassium recycling pathway in the thick ascending limb that sustains NKCC2-mediated sodium chloride reabsorption, and it mediates potassium secretion in the cortical collecting duct, placing it downstream of WNK kinase and SGK1 regulation. Loss-of-function mutations cause antenatal Bartter syndrome type 2 with salt wasting and, paradoxically, transient neonatal hyperkalemia, and mouse nulls reproduce polyuria, hydronephrosis, and high perinatal mortality on many backgrounds. Conditional alleles with nephron segment-restricted Cre drivers separate thick ascending limb from collecting duct contributions, while knockins of specific pH-sensing or PIP2-binding residues test gating mechanism, and humanized pore alleles support ROMK inhibitor diuretic programs.
Whole body loss of Kcnj1 gives the clearest readout when the question is whether the gene is required at all. Follow up tissue work can still move to a conditional allele if lethality or compensation appears.
What Kcnj1 Knockout mouse models are available?
ingenious targeting laboratory offers 1 distinct Kcnj1 knockout catalog mouse model. Researchers can order pre-developed catalog lines or request a custom mouse model, including humanized, knockin, and transgenic variations with verified germline transmission.
Catalog table
| Model | Type | Category | Availability | Catalog # | Action |
|---|---|---|---|---|---|
| Kcnj1-KO | Knockout | KO/CKO mice | embryo cryopreservation | KO 201290 | Inquire |
Why this approach
A conventional knockout answers whether the gene is required broadly. When your target tissue is the organ of interest, a global null can still be informative if viability is acceptable and you want the simplest genotype. If the null is harsh, a floxed allele with a regional Cre is the safer long term platform.
Pricing and quotes
The Kcnj1 Knockout lines listed above are catalog models. Send us the catalog number and our team confirms current availability, pricing, and whether the line ships cryopreserved or live.
If your study needs a Kcnj1 allele configuration that is not listed above, our scientific team designs and generates it. Model generation quotes return in about twenty four hours with project milestones and pricing.
FAQ
What Kcnj1 Knockout mouse models are available?
When Kcnj1 Knockout lines are in catalog, we ship from inventory. If your configuration is not listed, we design the allele to order. Common paths include conditional knockout, constitutive knockout, humanized, knockin, and transgenic options, with documented germline transmission and United States QC.
Is Kcnj1 knockout embryonic lethal in mice?
It depends on background and allele design. Some Kcnj1 germline knockouts are viable. Others need conditional alleles or mixed backgrounds. We review publications and our own experience, then recommend floxed versus null before you commit.
Which Cre driver is best for experiments?
Driver choice depends on onset timing, recombination efficiency, and known leak. We map your organ and cell type to a short list of proven Cre lines, then talk through reporter crosses and controls. We favor drivers with strong community validation for your tissue.
Do you ship live Kcnj1 animals?
When catalog lines are live, we ship with health certificates and QC documentation. If your exact combo is not listed, we quote a generation project with cryo or live dispatch depending on cohort timing and geography.
How do I request a quote for Kcnj1?
Use the catalog inquire buttons or the request quote form. Include your allele goal, Cre plan if any, strain background, and cohort size. A PhD led team responds with pricing, milestones, and the fastest path to experimental animals.